Cd9 clone ts9

Manufactured by Thermo Fisher Scientific

The CD9 (clone Ts9) is a laboratory equipment product from Thermo Fisher Scientific. It is a marker used to detect the presence of the CD9 protein, which is involved in various cellular processes. The product provides a tool for researchers to study CD9 expression and its role in different biological systems.

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Lab products found in correlation

Cd63 clone ts63, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Rabbit anti mouse hrp conjugated secondary antibody, by Agilent Technologies (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Gm130, by BD (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated polyclonal antibody goat anti rabbit, by Agilent Technologies (1 mentions)

2 protocols using cd9 clone ts9

Immunofluorescence Staining of Cellular Markers

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The following primary antibodies were applied in this study: mouse monoclonal anti-human intercellular adhesion molecule-1 (clone 15.2, Santa Cruz, sc-107), CD63 (clone Ts63, Thermo Fisher) and CD9 (clone Ts9, Life Technologies), GM-130 (610822, BD Biosciences), β-actin (Santa Cruz), Rabbit anti-mouse HRP-conjugated secondary antibody (Dako, P0260) and donkey anti-mouse IgG, Alexa Fluor^® 488 antibody (clone A-21202, Thermos Fisher). Calcein, AM (C3099a), CellMask™ orange plasma membrane stains (CS10045), and Hoechst 33342 were obtained from Thermo Fisher Scientific. 4, 6 diamidino-2-phenylindole (DAPI) was provided by Sigma-Aldrich.

Hosseinkhani B., Kuypers S., van den Akker N.M., Molin D.G, & Michiels L. (2018). Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Frontiers in Immunology, 9, 1789.

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Immunofluorescence Analysis of Cell Markers

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The following primary antibodies were used: mouse monoclonal anti-human ICAM-1 (clone 15.2, sc-107, Santa Cruz Biotechnology), Annexin II (clone C-10, sc-28385, Santa Cruz Biotechnology), CD63 (clone Ts63, Thermo Fisher), CD9 (clone Ts9, Life Technologies) and rabbit anti-BAX antibody (clone E63, ab32503, Abcam). Polyclonal rabbit anti-mouse HRP-conjugated antibody (DAKO), polyclonal goat anti-rabbit HRP-conjugated antibody (DAKO), donkey anti-mouse IgG, Alexa Fluor® 488 – antibody (clone A-21,202, Thermo Fisher) and Alexa Fluor® 488 anti-human ICAM-1 (clone 15.2, sc-107, AF488, Santa Cruz Biotechnology) were used as secondary antibodies.

Hosseinkhani B., van den Akker N.M., Molin D.G, & Michiels L. (2020). (Sub)populations of extracellular vesicles released by TNF-α –triggered human endothelial cells promote vascular inflammation and monocyte migration. Journal of Extracellular Vesicles, 9(1), 1801153.

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