According to the foregoing preparation of HASH-microgels, 3.56% (w/v) HASH was mixed on-chip with a solution of 3.77% (w/v) PEG-norb2 containing 0.25% (w/v) LAP. As-formed W/O emulsions were directly injected into an on-chip UV-chamber with a height of 20 µm and UV-polymerized (250–450 nm, 44.6 mW cm−2), while being retained in non-spherical shape. Egg-shaped HASH-0.75 microgels were prepared by setting the flow rates to 500 µL h−1 for the continuous phase and 30 µL h −1 for both dispersed phases. Disk like HASH-0.75 microgels were prepared by setting the flow rates to 300 µL h−1 for the continuous phase and 50 µL h−1 for both dispersed phases. The 3D reconstruction of x,y-microgel images and the evaluation of the corresponding microgel aspect ratios were performed using the Leica LAS X 3 D visualization software.
Las x 3 d visualization software
Manufactured by Leica
LAS X 3D visualization software is a tool for processing and analyzing three-dimensional data. It provides users with the ability to view, manipulate, and extract information from 3D images and models.
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Lab products found in correlation
2 protocols using las x 3 d visualization software
1
Fabrication of Egg-Shaped and Disk-Like HASH Microgels
2
Confocal Microscopy Imaging of Fluorescently Labeled Blood Vessels
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Cleared samples were imaged on an inverted Leica TCS SP8 confocal laser scanning microscope, using a 20×/0.75 HC PL Apo objective lens. The samples were positioned in a glass-bottom Petri dish and submerged in DBE. DAPI was visualized using a 405-nm diode laser, and the Alexa Fluor 546 fluorescence was imaged with the 546-nm wavelength of a white light laser. For each sample, an image stack (z step size ∼5 μm) with 1,024 × 1,024 pixel resolution was captured. Three-dimensional renderings were obtained using Leica LAS X 3D visualization software. The Imaris image analysis software enabled the specific selection and measurement of the IP blood vessels, by applying a new surface rendering based on the intensity of the fluorescent signal. Following the imaging, the samples were embedded in paraffin, cut into 5-μm sections, and stained with hematoxylin and eosin (H&E). H&E stains were imaged using an Olympus BX43 microscope.
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