The following antibodies were used in the experiments: allophycocyanin (APC)-conjugated mouse anti-CD44 from BD Biosciences, phycoerythrin (PE)-conjugated mouse anti-CD133 from Miltenyi Biotec, rabbit anti-CD44, rabbit anti-CD133, and mouse anti-OGT from Abcam, Alexa 647-conjugated rabbit anti-mouse from Invitrogen; rabbit anti-β-tubulin from Cell Signaling Technology (Danvers, MA, USA); Alexa 488-conjugated goat anti-rabbit from Molecular Probes, Inc., (Eugene, OR, USA), mouse anti-O-GlcNAc (RL2) from Thermo Fisher Scientific; mouse anti-GAPDH from Santa Cruz Biotechnoloigy Inc (Sta. Cruz, CA, USA).
Mouse anti o glcnac rl2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Mouse anti-O-GlcNAc (RL2) is a monoclonal antibody that recognizes the O-linked N-acetylglucosamine (O-GlcNAc) modification on proteins. It can be used to detect and study the presence of O-GlcNAc in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Alexa 488 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Apc conjugated mouse anti cd44, by BD (1 mentions)
Pe conjugated mouse anti cd133, by Miltenyi Biotec (1 mentions)
Rabbit anti β tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti cd44, by Abcam (1 mentions)
Rabbit anti cd133, by Abcam (1 mentions)
Rabbit anti actin, by Abcam (1 mentions)
T per tissue protein extraction reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti o glcnac rl2
1
Investigating Cancer Stem Cell Markers
2
Protein Expression and Western Blot Analysis
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Protein was extracted with T-PER tissue protein extraction reagent (Thermo-Fisher). Lysates were run on a 4–12% Bis-Tris gel and transferred to a nitrocellulose membrane. Membranes were blocked with Odyssey blocking buffer (Odyssey, number 927–40000) for 1 h, incubated with primary antibodies rabbit anti-actin (Abcam) and mouse anti-O-GlcNAc (RL2) (Thermo Fisher MA1-072) overnight at 4 °C. Secondary antibodies were Odyssey IR dye-conjugated. Membranes were imaged and band intensities were analyzed with Odyssey imaging equipment.
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