Goat anti guinea pig hrp conjugate

The primary antibodies used for immunoblotting were mouse anti-V5 (1:5000, Invitrogen), guinea pig anti-Axin (1:1000, [17 (link)]), rabbit anti-Kinesin Heavy Chain (1:10000, Cytoskeleton), mouse anti-alpha-Tubulin (1:10000, DM1A, Sigma), rabbit anti-alpha-Tubulin (1:10000, Sigma), rabbit anti-Gluthathione-S-Transferase (1:10000, Invitrogen), rabbit anti-phospho-LRP6 [Thr1572] (1:1000, Millipore), mouse anti-Nervana antibody (Nrv5F7, 1:1000, DSHB), and guinea pig anti-Arrow antibody (1:1000, [74 (link)]). The primary antibodies used for immunostaining were guinea pig anti-Axin (1:1000, [17 (link)]), rabbit anti-β-gal (1:1000; MP Biomedicals), mouse anti-Arm (1:20; DSHB), mouse anti-Fas III (1:20; DSHB), mouse anti-Discs Large (1:20; DSHB), rabbit anti-GFP (1:200; Invitrogen), mouse anti-V5 (1:5000; Invitrogen), rabbit anti-Apc2 (1:1000; [75 (link)]), and guinea pig anti-Senseless (1:1000, [76 (link)]).
The secondary antibodies used for immunoblotting were: goat anti-rabbit HRP conjugate (1:10000, Biorad), goat anti-mouse HRP conjugate (1:10000, Biorad), and goat anti-guinea pig HRP conjugate (1:10000, Jackson ImmunoResearch). The secondary antibodies used for immunostaining were goat or donkey Alexa Fluor 488, 555 or Cy5 conjugates (1:400; Invitrogen).

The primary antibodies used for immunostaining were mouse anti-V5 (1:5000; Invitrogen), mouse anti-Wingless (1:200, 4D4 concentrated antibody, Developmental Studies Hybridoma Bank, DSHB). The secondary antibodies used for immunostaining were goat or donkey Alexa Fluor 488 or 555 conjugates (1:400; Invitrogen). The primary antibodies used for immunoblotting were mouse anti-V5 (1:5000, Invitrogen), guinea pig anti-Axin (1:1000, [54 (link)]), rabbit anti-Kinesin Heavy Chain (1:10000, Cytoskeleton), mouse anti-Arm (1:100, N2 7A1, DSHB), mouse anti-alpha-Tubulin (1:10000, DM1A, Sigma), rabbit anti-alpha-Tubulin (1:10000, Sigma), rabbit anti-Gluthathione-S-Transferase (1:10000, Invitrogen), guinea pig anti-Apc2 (GP10) 1:5000 [44 (link)]; rabbit anti-phospho-LRP6 [Thr1572] (1:1000, Millipore), rat anti-Dishevelled (1:1000, [70 (link)]), and guinea pig anti-Arrow (1:1000, [71 (link)]). The secondary antibodies used for immunoblotting were: goat anti-rabbit HRP conjugate (1:10000, Biorad), goat anti-mouse HRP conjugate (1:10000, Biorad), and goat anti-guinea pig HRP conjugate (1:10000, Jackson ImmunoResearch).