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2 protocols using estriol

1

Solubilization of Diverse Pharmaceutical Compounds

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The drugs (1 wt%) were added to the solvents and stirred over 10 h at room temperature. When the drugs were not solubilised, the solutions were heated up to 80 °C and stirred for 2 h. We checked whether the drugs were solubilised by observing the particles of the drugs using an invert microscope (ECLIPSE Ts2, Nikon Corporation). Erythromycin, 3′-azido-3′-deoxythymidine, 5-iodo-2′-deoxyuridine, (+)-catechin hydrate, l-adrenaline, desloratadine, zoledronic acid monohydrate, testosterone, estriol, and oxaliplatin were purchased from Tokyo Chemical Industry Co., Ltd and used as received. Fluorouracil, carboplatin and cisplatin were purchased from Fujifilm Wako Pure Chemical Corporation and used as received. Adenosine 3′-phosphate and insulin were purchased from Nacalai Tesque Inc. and used as received. l-Thyroxine was purchased from Sigma-Aldrich Co., Llc. and used as received. Paclitaxel was purchased from Funakoshi Co., Ltd and used as received.
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2

Halophenols and EDCs Analysis Protocol

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For halophenols analysis, 2,4,6-trichlorophenol, 2,4,6-tribromophenol, 3,5-dichloro-4-hydroxybenzoic acid, 3,5-dichlorosalicylic acid, 3,5-dibromo-4 -hydroxybenzoic acid, and 3,5-dibromosalicylic acid were purchased from Sigma-Aldrich. The EDCs standards, including estrone (E1, 99%), 17β-estradiol (E2, 97.0%), estriol (E3, 99.0%) and bisphenol A (BPA, 99.9%) were purchased from Tokyo Chemical Industry, and 17α-ethinyl estradiol (EE2, 98%) purchased from Sigma-Aldrich. Isotopic labelled bisphenol A-d16 (BPA-d16, 98% from Sigma-Aldrich) was used as the internal standard for LC-MS/MS analyses of EDCs and halophenols.
The E. coli strain (ATCC 8099) was purchased and cultivated in the lab to prepare the E. coli suspension following the method described by Wu et al. (2018) . Unless otherwise noted, other chemicals were used at least regent grade purity, and all solutions were prepared in ultrapure water (18.2 MΩ/cm).
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