Lunar idxa dual energy x ray absorptiometer

Blood samples were immediately placed on ice, centrifuged at 4°C, separated, and stored at −80°C. For blood drawn during the OGTT, plasma glucose was analysed using a YSI analyser (Yellow Springs, Ohio), plasma insulin was measured by chemiluminescence using the Access Ultrasensitive Immunoenzymatic assay system (Beckman Coulter, Chaska, Minnesota),²⁹ C‐peptide concentrations were measured using a radioimmunoassay (Linco Research, St Charles, Missouri),²⁹ and plasma [6,6‐²H₂] glucose enrichment was measured using gas chromatographic mass spectrometry.²⁸For blood drawn during the triple‐tracer cortisol test, plasma cortisol, cortisone, ²H cortisol, ¹³C cortisone, ¹³C cortisol and ³H cortisol radioactivity were measured using methods previously described.⁹, ³⁰Body composition (ie, total body fat and abdominal fat measurement), were measured using the Lunar iDXA dual‐energy X‐ray absorptiometer, software version 6.10 (GE Healthcare, Madison, Wisconsin). LFF was quantified and liver fibrosis staged with MRE.²¹, ²², ²³, ²⁴, ³¹, ³², ³³, ³⁴, ³⁵, ³⁶