CRFK cells infected with FPV isolate (MOI = 0.1) in 96-well plates were treated with cold 80% (v/v) acetone for 15 min, washed with PBS three times, exposed to a mouse anti-CPV antibody (Abcam, Cambridge, United Kingdom) at 37°C for 1 h, and then treated with an Alexa Fluor 488-conjugated goat anti-mouse IgG H&L antibody (Abcam, Cambridge, United Kingdom). Then, diamidino-2-phenylindole (DAPI) (Solarbio, Beijing, China) dye was added for fluorescent staining of cell nuclei. FPV infected cells were observed under a fluorescence microscope (ZEISS, Oberkochen, Germany) after being washed with PBS; cells evidencing intranuclear fluorescence were considered infected with FPV.
Mouse anti cpv antibody
Manufactured by Abcam
Sourced in United Kingdom
The Mouse anti-CPV antibody is a laboratory reagent used for the detection and identification of canine parvovirus (CPV) in samples. It is designed to specifically bind to the CPV antigen, allowing for its detection and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Diamidino 2 phenylindole dapi, by Solarbio (1 mentions)
Fluorescence microscope, by Zeiss (1 mentions)
Alexa fluor 488 conjugated goat anti mouse igg h l antibody, by Abcam (1 mentions)
Dmi4000b microscope, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti cpv antibody
1
Fluorescent Detection of FPV Infection
2
Immunofluorescence Staining of CPV
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Cells were washed with PBS, fixed with 4 % paraformaldehyde for 15 min, and permeabilized with 0.2 % Triton X-100 for 10 min. After washing three times with PBS, the cells were incubated with mouse anti-CPV antibody (1:2000) (Abcam, ab140431, Britain) for 1 h. Subsequently, FITC-conjugated goat anti-mouse IgG (1:500) (Zhongshan, China) was used as the secondary antibody. As a reference protein, nuclear staining was done with 4′,6-diamidino-2-phenylindole (DAPI) according to instructions of the manufacturer (Invitrogen, Carlsbad, CA, USA). Finally, fluorescence was observed under a Leica DMI4000 B microscope (Leica, Wetzlar, Germany).
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