Rosiglitazone rgz

TBT was obtained from Tokyo Chemical Industry (Tokyo, Japan) and dissolved in dimethyl sulfoxide (DMSO). Rosiglitazone (RGZ), 2-mercaptoethanol (2-ME), and sodium butyrate (NaB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Y-27632, SB431542, LDN193189, and CHIR99021 were obtained from Wako (Tokyo, Japan). The penicillin-streptomycin mixture (PS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). U0126 was obtained from Enzo Life Sciences (Farmingdale, NY, USA). All other reagents were of analytical grade and were obtained from commercial sources.

Procyanidin B2 (PCB2) was purchased from MedChem Express (Monmouth Junction, NJ, USA). Rosiglitazone (RGZ) and GW9662 were purchased from Sigma-Aldrich (St. Louis, MO, USA). GW6471 and GSK0660 were purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Anti-CD206 and anti-CD86 PE and PE-conjugated rat IgG2a, κ were from Biolegend (San Diego, CA, USA). Rabbit polyclonal antibodies against Ym1 and Fizz1 were purchased from Abcam (Cambridge, MA, USA). Phosphorylated PPARγ (Ser112) rabbit polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). PPARγ antibody was from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-actin, Arg1, and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).