Twenty µg of protein lysate were separated by SDS-PAGE. PVDF membranes were blocked by 0.1% TBS-Tween - 5% milk. Primary antibodies were incubated overnight at 4 °C. The antibodies used in western blot were: Galectin-3 (Abcam ab2785), Integrin β1 (Millipore AB1952), Integrin α6 (Abcam ab75737), FAK (Abcam ab40794), phospho-FAK Y397 (Abcam ab81298) Talin1/2 (Abcam ab11188), Calpain-2 (Abcam ab155666), Piezo 1 (Proteintech 15939-1-AP) and GAPDH (Millipore MAB374) as a loading control. Horseradish peroxidase-coupled secondary antibodies (Cell Signalling) were incubated 2 hours at room temperature. The Chemidoc XRS+ imager was used for chemiluminescence detection. Pixel quantifications were done with Image Lab software. Normalization by sum using total protein staining was used.
Phospho fak y397
Manufactured by Abcam
Sourced in United States
Phospho-FAK Y397 is a primary antibody that detects the phosphorylated form of Focal Adhesion Kinase (FAK) at the tyrosine 397 residue. Phosphorylation of this site is a crucial event in the activation of FAK, a key regulator of cell adhesion and migration.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase coupled secondary antibody, by Cell Signaling Technology (1 mentions)
Gapdh, by Merck Group (1 mentions)
Integrin α6, by Abcam (1 mentions)
Integrin β1, by Merck Group (1 mentions)
Galectin 3, by Abcam (1 mentions)
Calpain 2, by Abcam (1 mentions)
Piezo1, by Proteintech (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Pe annexin 5 apoptosis detection kit, by BD (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
Atg13, by Cell Signaling Technology (1 mentions)
Phospho beclin 1 s15, by Abbiotec (1 mentions)
Azd8055, by Active Motif (1 mentions)
Glucose free media, by Thermo Fisher Scientific (1 mentions)
Ampk alpha, by Cell Signaling Technology (1 mentions)
Paxillin, by BD (1 mentions)
Total erk1 2, by Cell Signaling Technology (1 mentions)
Total acc, by Cell Signaling Technology (1 mentions)
P p90rsks380, by Cell Signaling Technology (1 mentions)
4 protocols using phospho fak y397
1
Protein expression analysis by Western blot
2
Autophagy Signaling Pathway Analysis
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Signaling Pathway Antibody Analysis
4
Protein Expression Analysis via SDS-PAGE
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An analytical 10% sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) was performed, and 30 μg of protein was analyzed, unless stated otherwise. For immuno-blotting, proteins in the SDS gels were transferred onto a polyvinylidene difluoride membrane by an electroblot apparatus. Antibodies against ARNT (Cell Signaling Technology, Inc., Danvers, MA, USA), α-tubulin (Sigma Corporation, Cream Ridge, NJ, USA), N-cadherin, β-catenin, E-cadherin, vimentin, fibronectin, integrin β1, and phospho-FAKY397 (Epitomics, Inc., Burlingame, CA, USA), NQO1 (Proteintech Group Inc., W Campbell Park, Chicago, USA), Complex I subunit (NDUFB8), Complex II subunit (SDHB), Complex III subunit (UQCRC2), Complex IV subunit II (COX II), and ATP synthase subunit alpha (ATP5A) as an optimized premixed cocktail (Abcam Inc., Cambridge, UK) were used as the primary antibodies. Mouse or rabbit IgG antibodies coupled to horseradish peroxidase were used as secondary antibodies. An enhanced chemiluminescence kit (Pierce, Rockford, IL) was used for detection.
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