A larval visual motor response test was performed at 120 hpf to determine if F1 larvae exhibited altered behavior from parental ATZ exposure following an established protocol (Horzmann et al. 2018 (link), Ahkin Chin Tai et al. 2021 (link)). Each larvae was individually placed into a 96 square well plate in 0.5 mL of aquaria water. Larvae were acclimated for 10 min at 28 °C in the Noldus DanioVision Observation Chamber (Noldus Information Technology, Wagenningen, Netherlands). The Noldus white light routine consisting of alternating 10 min of dark and white light to test the visual motor response was performed over 50 min (3 dark phases and two light phases). Behavioral experiments were performed between 11 am to 1 pm to minimize circadian variability in movement. Infrared movement was recorded during behavioral experiments at a rate of 25 frames per second with a Basler GenICam acA 1300–60 g camera and analyzed with Noldus EthoVision 12.0 software. Tracks were smoothed for total distance moved, velocity, and time spent moving. Each treatment group (0, 0.3, 3, or 30 ppb ATZ F1) had 8 biological replicates containing 24 subsample fish per biological replicate for a total of 192 fish per treatment group.
Genicam aca 1300 60 g camera
Manufactured by Basler
The GenICam acA 1300–60 g camera is a high-performance industrial camera from Basler. It features a 1.3 megapixel sensor and can capture images at a frame rate of up to 60 frames per second. The camera supports the GenICam standard for easy integration with a variety of software and hardware systems.
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Lab products found in correlation
2 protocols using genicam aca 1300 60 g camera
1
Larval Visual Motor Response to ATZ
2
Quantifying Spontaneous Movement and Photoperiod Response in Larval Zebrafish
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At 24 hpf, six of live embryos in each group were selected randomly to count the times of spontaneous movement (SM, free swimming activity independent of any stimuli) within 1 min under microscope. Experiment was repeated at least three times.
At 120 hpf, a total of 12 healthy larvae randomly selected from each group were transferred into 96-well plates with one larva and 200 μL E3 embryo medium per well, which was placed in the Noldus DanioScope Observation Chamber (Noldus Industrial Co., Shanghai, China). After 10 min of acclimatization in the chamber, SM was monitored within 10 min with a Noldus EthoVision system (Noldus Industrial Co., Shanghai, China) at 28 °C. Besides, larva response to photoperiod stimulation was tracked during a cycle of two alternating periods of 10 min light (LED illumination) and 10 min dark (infrared illumination), after 10 min of acclimatization too. Using a Basler GenICam acA 130,060 g camera, the infrared movement traces were recorded at a rate of 25 frames per second and analyzed with the Noldus EthoVision XT 15 software. The total moving distance and average speed were determined during dark and light periods, respectively. Experiment was repeated at least three times.
At 120 hpf, a total of 12 healthy larvae randomly selected from each group were transferred into 96-well plates with one larva and 200 μL E3 embryo medium per well, which was placed in the Noldus DanioScope Observation Chamber (Noldus Industrial Co., Shanghai, China). After 10 min of acclimatization in the chamber, SM was monitored within 10 min with a Noldus EthoVision system (Noldus Industrial Co., Shanghai, China) at 28 °C. Besides, larva response to photoperiod stimulation was tracked during a cycle of two alternating periods of 10 min light (LED illumination) and 10 min dark (infrared illumination), after 10 min of acclimatization too. Using a Basler GenICam acA 130,060 g camera, the infrared movement traces were recorded at a rate of 25 frames per second and analyzed with the Noldus EthoVision XT 15 software. The total moving distance and average speed were determined during dark and light periods, respectively. Experiment was repeated at least three times.
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