Extracellular recordings from custom tetrodes were recorded by a OmniPlex D Neural Acquisition System (Plexon). Each channel was amplified and bandpass filtered (154 Hz–8.8 kHz) to obtain both single-unit spike activity and LFPs (1.5 Hz–400 Hz). Spike channels were locally referenced to remove both movement-related noise and potential electrical noise. Spikes were detected via threshold crossing and digitized at 40 kHz. To isolate single-units, waveform clusters from all four electrodes within a tetrode were manually identified using the Offline Sorter v3 (Plexon). Cineplex Studio (Plexon) was used for capturing animal location data via three infrared LEDs positioned atop the surgically implanted microdrive. Cineplex Editor (Plexon) was employed offline to enter event markers and to verify animal position data.
Omniplex d neural acquisition system
Manufactured by Plexon
The OmniPlex D Neural Acquisition System is a data acquisition system designed for recording neural signals. It offers multi-channel recording capabilities and supports a range of electrode types. The system is capable of acquiring and processing neural data, but a detailed description of its core function is not available without potentially introducing bias or interpretation.
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Lab products found in correlation
2 protocols using omniplex d neural acquisition system
1
Extracellular Recordings and Position Tracking
2
Electrophysiological Recordings and Neural Tracking
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Electrophysiological recordings for this project were collected using a 96 channel OmniPlex D Neural Acquisition System (Plexon). Each channel was amplified and bandpass filtered for both single-unit activity (154 Hz to 8.8 kHz) and local field potentials (1.5 Hz to 400 kHz). Spike channels were referenced to a local electrode in the same region in order to remove both movement-related and any electrical noise. Action potentials of neurons were detected via threshold crossing and digitized at 40 kHz. Between recorded training sessions tetrodes were advanced based on visual inspection, in order to maximize neural unit yield, and allowed to settle overnight before conducting the next recording session. Individual neural units were isolated via manual offline clustering, employing Offline Sorter v3 (Plexon). Cineplex Studio (Plexon) was used for capturing behavioral tracking data via three infrared LEDs positioned atop the microdrive EIB. Cineplex Editor (Plexon) was employed to enter event markers and to verify animal positional tracking data.
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