Matrigel invasion assay was performed using Transwells containing 8.0 µm pore membranes (Corning, Lowell, MA, USA). Cells were placed in the upper chamber of the Transwell for 48 h. Then the chambers were washed twice with PBS. The lter side of the upper chamber was cleaned with a cotton swab. Next, the remained cells in the upper chamber of the membrane were cut out of the insert. Cells were xed in methanol and stained with 5% Giemsa for 30 min at room temperature. The invaded cells were photographed and counted under a light microscope.
Transwells containing 8.0 m pore membranes
Manufactured by Corning
Sourced in United States
Transwells containing 8.0 µm pore membranes are a laboratory product manufactured by Corning. The core function of this product is to provide a permeable support for cell culture experiments.
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Lab products found in correlation
2 protocols using transwells containing 8.0 m pore membranes
1
Matrigel Invasion Assay Protocol
2
Matrigel Invasion Assay Protocol
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Matrigel invasion assay was performed using Transwells containing 8.0 µm pore membranes (Corning, Lowell, MA, USA). Cells were placed in the upper chamber of the Transwell for 48 h. Then the chambers were washed twice with PBS. The lter side of the upper chamber was cleaned with a cotton swab. Next, the remained cells in the upper chamber of the membrane were cut out of the insert. Cells were xed in methanol and stained with 5% Giemsa for 30 min at room temperature. The invaded cells were photographed and counted under a light microscope.
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