Methanol, acetonitrile (both HPLC gradient grade), toluene, acetone, ethanol, ethyl acetate, n-hexane, and formic acid were purchased from Merck (Darmstadt, Germany). Water was purified by Milli-Q plus system from Millipore (Molsheim, France). Trisodium citrate dihydrate and disodium hydrogencitrate sesquihydrate were purchased from Sigma-Aldrich (Madrid, Spain) while NaCl was purchased from Fischer. Primary secondary amine bonded silica (PSA) was acquired from Supelco (Supelclean™, Bellefonte, PA, USA). Anhydrous magnesium sulfate was purchased from Fluka. Ammonium formate was acquired from VWR. Pesticide standards and internal standard (triphenylphosphate-TPP and dinitrocarbanilide or 1,3-bis(4-nitrophenyl)urea-DNC) were purchased from Sigma–Aldrich (Madrid, Spain) and were dissolved in toluene, acetone, ethanol, ethyl acetate, Methanol, n-hexane, or acetonitrile, depending on the solubility of the compound, at a concentration of 5 mg/L. These stock solutions were subsequently used to prepare different working solutions for calibrations. Working solutions were prepared in acetonitrile. All standard solutions were stored in amber vials in the dark at −20 °C, for at least 3 years [20 ], and before use, they were kept at room temperature for 15 min.
Disodium hydrogen citrate sesquihydrate
Manufactured by Merck Group
Sourced in Spain, Germany
Disodium hydrogen citrate sesquihydrate is a chemical compound used as a buffer in various laboratory applications. It is a white crystalline solid that acts as a pH regulator, maintaining a specific acidity or basicity in solutions. The compound is soluble in water and is commonly used in the preparation of buffers and in the control of pH in analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Ammonium formate, by Avantor (2 mentions)
Milli q plus system, by Merck Group (2 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Trisodium citrate dihydrate, by Merck Group (1 mentions)
Dinitrocarbanilide, by Merck Group (1 mentions)
Triphenyl phosphate tpp, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Primary secondary amine bonded silica psa, by Merck Group (1 mentions)
Anhydrous sodium sulphate, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
Hexadecane, by Merck Group (1 mentions)
N pentane, by Merck Group (1 mentions)
5 protocols using disodium hydrogen citrate sesquihydrate
1
Pesticide Residue Analysis Protocol
2
Lipid Extraction and FAME Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents and chemicals used for lipid extraction and FAME preparation were of analytical grade. Chloroform, methanol, n-hexane, ethanol, n-pentane, ammonia, diethyl ether, anhydrous sodium sulphate, disodium hydrogen citrate sesquihydrate, potassium hydroxide and sodium hydroxide, and the hexadecane (used as internal standards) were from Sigma Aldrich (Darmstadt, Germany). The standard mix of 37 FAMEs and standard mix of C4:0–C24:0 even-carbon saturated FAMEs were purchased from Supelco (Bellefonte, PA, USA).
3
Enzymatic Hydrolysis of Porcine Pancreatic α-Amylase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Porcine pancreas α‐amylase (E.C. 3.2.1.1) (A6255 type I‐A approx. 1,050 units/mg protein in saline solution, 29 mg proteins/ml), pepsin from gastric porcine mucosa (P7000), and protease type XIV isolated from Streptomyces griseus (P5147) were purchased from Sigma‐Aldrich. Amyloglucosidase from Aspergillus niger (EC 3.2.1.3., 3,300 units/ml on soluble starch, stabilized liquid in 50% (v/v) glycerol, 0.02% sodium azide) and isoamylase from Pseudomonas sp. (EC 3.2.1.68, 500 U/ml in ammonium sulfate suspension, 0.02% sodium azide) and a glucose assay kit based on enzymatic method (Mutarotase‐GOD) were purchased from Megazyme International Ireland Ltd. Standards of fatty acid methyl esters (FAMEs), methanol, KOH, methane sulfonic acid, tritridecanoin, sodium methoxide, and disodium hydrogen citrate sesquihydrate were obtained commercially from Sigma‐Aldrich. The other chemicals were reagent grade and obtained from Sigma‐Aldrich.
4
Simultaneous Mycotoxin Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol (MeOH) and acetonitrile (MeCN) of LC-MS grade, and ammonium formate were supplied by VWR International Eurolab S.L. (Barcelona, Spain). Formic acid eluent additive for LC–MS was obtained from Sigma Aldrich (St. Louis, MO, USA). Magnesium sulphate (MgSO4), sodium chloride (NaCl) and sodium citrate were purchased from Panreac Química (Barcelona, Spain), while disodium hydrogen citrate sesquihydrate was supplied by Merck (Darmstadt, Germany). Ultrapure water was obtained from a Milli-Q Plus system (Millipore Bedford, MA, USA).
Mycotoxin standard solutions (10 mg/L in MeCN) of OTA, STE, F-X, DON, CIT, ZEN, FB1, FB2, T-2 and HT-2 were purchased from Techno Spec (Barcelona, Spain). Individual standards (powder) of ENNA, ENNA1, ENNB, ENNB1 and BEA were obtained from Sigma Aldrich and stock solutions were prepared at 1000 mg/L in MeCN. Multi-mycotoxins intermediate working solutions in MeCN (1 mg/L of OTA and STE; 2 mg/L of CIT; 10 mg/L of FB1, FB2, T-2, HT-2 and ZEN; 100 mg/L of DON, ENNA, ENNA1, ENNB, ENNB1 and BEA and 1000 mg/L of F-X) were prepared by combining suitable aliquots of each individual standard stock solution. These solutions were stored at −20 °C.
Nylon syringe filters (13 mm, 0.22 μm, from VWR) were used for filtration of extracts prior to the injection into the chromatographic system.
Mycotoxin standard solutions (10 mg/L in MeCN) of OTA, STE, F-X, DON, CIT, ZEN, FB1, FB2, T-2 and HT-2 were purchased from Techno Spec (Barcelona, Spain). Individual standards (powder) of ENNA, ENNA1, ENNB, ENNB1 and BEA were obtained from Sigma Aldrich and stock solutions were prepared at 1000 mg/L in MeCN. Multi-mycotoxins intermediate working solutions in MeCN (1 mg/L of OTA and STE; 2 mg/L of CIT; 10 mg/L of FB1, FB2, T-2, HT-2 and ZEN; 100 mg/L of DON, ENNA, ENNA1, ENNB, ENNB1 and BEA and 1000 mg/L of F-X) were prepared by combining suitable aliquots of each individual standard stock solution. These solutions were stored at −20 °C.
Nylon syringe filters (13 mm, 0.22 μm, from VWR) were used for filtration of extracts prior to the injection into the chromatographic system.
5
Analytical Method Development for Pesticides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All analytes and the deuterated internal standards azoxystrobin-d4, chlorpyrifos-d10, and pyrene-d10 (all >98%) were purchased from BLDpharm (Kaiserslautern, Germany), ChemPUR (Karlsruhe, Germany), EDQM (Strasbourg, France), HPC Standards GmbH (Cunnersdorf, Germany), Merck (Darmstadt, Germany), or Tokyo Chemical Industries (TCI, Tokyo, Japan).
Acetonitrile (ACN) in HPLC grade was purchased from VWR (Darmstadt, Germany). 3-Ethoxy-1,2-propanediol (98%) was obtained from BLDpharm (Kaiserslautern, Germany). L-Gluconic acid γ-lactone (95%), D-sorbitol (99%), sodium citrate dihydrate (≥99%), and disodium hydrogen citrate sesquihydrate (≥99%) were purchased from Merck (Darmstadt, Germany). Shikimic acid (≥98%) was purchased from Carl Roth (Karlsruhe, Germany). SPE bulk sorbents of ethylenediamine-N-propyl-functionalized silica (PSA), octadecyl functionalized silica (C18), and graphitized carbon black (GCB) were obtained from Agilent Technologies (Santa Clara, CA, USA). The bulk of multi-walled carbon nanotubes (MWCNT, 95%) with an outside diameter of 20–30 nm was obtained from abcr (Karlsruhe, Germany). Anhydrous magnesium sulphate (MgSO4, ≥98%) was purchased from Grüssing (Filsum, Germany). Sodium chloride (NaCl, p.a.) was obtained from Bernd Kraft (Duisburg, Germany).
Acetonitrile (ACN) in HPLC grade was purchased from VWR (Darmstadt, Germany). 3-Ethoxy-1,2-propanediol (98%) was obtained from BLDpharm (Kaiserslautern, Germany). L-Gluconic acid γ-lactone (95%), D-sorbitol (99%), sodium citrate dihydrate (≥99%), and disodium hydrogen citrate sesquihydrate (≥99%) were purchased from Merck (Darmstadt, Germany). Shikimic acid (≥98%) was purchased from Carl Roth (Karlsruhe, Germany). SPE bulk sorbents of ethylenediamine-N-propyl-functionalized silica (PSA), octadecyl functionalized silica (C18), and graphitized carbon black (GCB) were obtained from Agilent Technologies (Santa Clara, CA, USA). The bulk of multi-walled carbon nanotubes (MWCNT, 95%) with an outside diameter of 20–30 nm was obtained from abcr (Karlsruhe, Germany). Anhydrous magnesium sulphate (MgSO4, ≥98%) was purchased from Grüssing (Filsum, Germany). Sodium chloride (NaCl, p.a.) was obtained from Bernd Kraft (Duisburg, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!