Human pool b v3

MicroRNA TaqMan^® Arrays (Thermofisher Scientific, Waltham, MA, USA) were performed as previously described [106 (link)]. Briefly, 300 ng of total RNA isolated from each of the three biological replicates of U87MG, transfected for 24 h with the vector pcDNA3.1_circSMARCA5 or with NC, were reverse-transcribed into specific cDNAs of 748 microRNAs using the TaqMan™ MicroRNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA, USA) and the Megaplex™ RT Primers Human Pool A v 2.1 and Pool B v 3.0 (ThermoFisher Scientific, Waltham, MA, USA). The products of Megaplex™ reactions were then pre-amplified using the following kits: Megaplex™ PreAmp Primers, Human Pool A v. 2.1, and Human Pool B v. 3.0 (ThermoFisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. The Megaplex™ PreAmp product of each sample was then loaded into an independent TaqMan^® Array MicroRNA Card A (in the case of samples reverse-transcribed and pre-amplified with pool A primers) or B (with samples reverse-transcribed and pre-amplified with pool B primers) (ThermoFisher Scientific, Waltham, MA, USA). PCR was performed using a QuantStudio™ 5 Real-Time PCR System (ThermoFisher Scientific, Waltham, MA, USA) (Figure S12).