In this study, we work on human MCF7 breast cancer cells (ATCC® HTB-22), human PC3 prostate cancer cell line (ATCC® CRL-1435), A375 human skin cancer cell line (ATCC® CRL-1619), PANC1 pancreatic cell line (ATCC® CRL-1469), A549 lung cancer cell line (ATCC® CCL-185) and colorectal cancer cell line CACO2 (ATCC HTB-37).[33 (link)] All of the cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose containing 10% Fetal Bovine Serum (FBS) (Bio Whittaker, Verviers, Belgium), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Buffer (10 mM), gentamicin (50 μg/mL), L-glutamine (100 μg/mL), streptomycin (100 μg/mL), penicillin (100 μg/mL), (Sigma, St. Luis, MO, USA). Trypsin-EDTA (Biowest, USA) was routinely used for subcultures. Cell growth was accomplished at 37°C in a 5% carbon dioxide 95% air atmosphere in CO2 incubator (EuroClone, Italy). Sulforhodamine B[33 (link)] was from Santa Cruz Biotechnology, Inc. Texas (USA). 3-(4,5-dimethylthiazol-2-yl)−2,5- diphenyltetrazolium bromide (MTT) was from (Sigma, USA).
Co2 incubator
Manufactured by Euroclone
Sourced in Italy
The CO2 incubator is a laboratory equipment designed to provide a controlled environment for cell culture growth. It maintains a stable temperature and CO2 concentration, ensuring optimal conditions for cultivating cells.
Automatically generated - may contain errors
Lab products found in correlation
Sulforhodamine b, by Santa Cruz Biotechnology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Trypsin edta, by Biowest (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes buffer, by Merck Group (1 mentions)
Htb 22, by American Type Culture Collection (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
2 protocols using co2 incubator
1
In Vitro Evaluation of Anti-Cancer Potential
2
Zebrafish Scale Culture and TRAP Assay
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In accordance with our method recently described (Pasqualetti et al. 2012a,b) , explanted scales from AB zebrafish were cultured in fresh Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum, l-glutamine and 2% penicillin-streptomycin mixture in a CO 2 incubator (Euroclone, Italy) at 28°C under 5% CO 2 . In the treated group, prednisolone was added to a final concentration of 50 μM. At the end of the treatment, each group of scales was washed with phosphate-buffered saline (PBS), transferred as single scale per well in a 96-well microplate and processed for the evaluation of TRAP activity at three different time points, 24, 48, 72 h according with the method previously described and at 96 h to confirm the decrease of cells viability (Pasqualetti et al. 2012a,b) . The medium was changed every 24 h.
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