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2 protocols using 2 propanal

1

Magnetic Bead-Based Nucleic Acid Amplification

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Tetraethyl orthosilicate (TEOS), AO, PAA (MW ~56,000), PSS (MW ~70,000), avidin, were purchased from Sigma-Aldrich, USA. MB, 25% ammonia solution, 2-propanal were purchased from Merck, Germany. DynabeadsTM MyOneTM Streptavidin T1 was purchased from Thermo Fisher Scientific, USA. RCA reagents (deoxyribonucleotide triphosphate (dNTPs), phi29 DNA polymerase were purchased from Integrated DNA technologies, USA. MagLEAD Consumable Kit and magLEAD 12gC instrument were purchased from Precision System Science, Chiba, Japan. For electrochemical measurements, two-electrode screen-printed carbon electrodes (SPCE) were obtained from Quasense, Thailand and the PalmSens4 potentiostat with PSTrace software was obtained from Palmsens, The Netherlands. All the reagent and buffer solutions were prepared with deionized (DI) water (18.2 MΩ).
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2

Chitin-Chitosan Powder Characterization

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The food grade CS powder with 200 mesh particle size had molecular weight of 8.97 × 105 DA and 80% degree of deacetylation. It was purchased from Sinudom Agriculture Products Co., Ltd. (Surat Thani, Thailand). NI was provided by Shandong Freda Biotechnology Co., Ltd. (Shandong, China). CO (Syzygium aromaticum, Lin), with eugenol content in 70–80% range (manufacturer's data) was purchased from Thai China Flavors and Fragrances Industry Co., Ltd. (Bangkok, Thailand). The chemical agents used were analytical grade: chloroform, dipotassium hydrogen phosphate, phenolphthalein, methanol, potassium iodide, trichloroacetic acid, hydrochloric acid, 2-propanal, sodium hydroxide, sodium thiosulfate, thiobarbituric acid, potassium dihydrogen phosphate, anhydrous sodium sulfate, (Merck, Darmstadt, Germany), acetic acid (Lab-Scan, Bangkok, Thailand), glycerol (Vidhyasom, Thailand), Tween 80 (Labchem, Australia), palmitic acid, and 1,1,3,3-tetramethoxypropane (Sigma-Aldrich, St. Louis., MO, USA). The media for microbiological analyses were analytical grade: plate count agar; peptone; deMan, Rogosa, and Sharpe (MRS) agar; and violet red bile glucose agar (VRBG) (Merck, Darmstadt, Germany).
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