Fmr1 KO (Fmr1-/y) and X-linked GFP mice were obtained from Jackson laboratories (Stock No: 003025 and 003116, respectively). Fmr1fl/fl were obtained from Dr. David Nelson (Baylor College of Medicine) (Mientjes et al., 2006 (link)). Mice were maintained on a C57BL/6 J background and reared on a 12 hr light-dark cycle with access to food and water ad libitum. Male Fmr1 WT (Fmr1+/y) with Fmr1 KO (Fmr1-/y) littermates were used for experiments and both male and female pups were used for Fmr1 flox (Fmr1fl/y and Fmr1fl/fl). All animal experiments were conducted in accordance with the Institutional Animal Care and Use Committee (IACUC) at University of Texas Southwestern Medical Center.
X linked gfp mice
Manufactured by Jackson ImmunoResearch
The X-linked GFP mice are a transgenic mouse line that expresses green fluorescent protein (GFP) under the control of the X-linked phosphoglycerate kinase (PGK) promoter. This results in the expression of GFP in all cells of the body, making these mice useful for various research applications that require the visualization of cells or tissues.
Automatically generated - may contain errors
2 protocols using x linked gfp mice
1
Generation and Maintenance of Fmr1 Mouse Models
2
Viral Manipulation of Fmr1 Mouse Model
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Fmr1 KO (Fmr1 -/y ) and X-linked GFP mice were obtained from Jackson laboratories (Stock No: 003025 and 003116, respectively). Fmr1 fl/fl were obtained from Dr. David Nelson (Baylor College of Medicine) (Mientjes et al., 2006) . Mice were maintained on a C57BL/6J background and reared on a 12 hr lightdark cycle with access to food and water ad libitum. Male Fmr1 WT (Fmr1 +/y ) with Fmr1 KO (Fmr1 -/y ) littermates were used for experiments and both male and female pups were used for Fmr1 flox (Fmr1 vg/mL using sterile saline. Traces of Fast Green FCF dye (Sigma) were added to facilitate visualization of virus spreading. Neonatal mouse pups (P1) were first anesthetized by hypothermia, then fixed on a customized mold and placed on a stereotaxic frame. AAV9.eGFP-Cre (420-560 nL) was delivered to left ventricle at a depth of approximately 1.1 mm underneath skull through a beveled glass pipette using Nanoject II injector (Drummond Scientific, Inc.). AAV9.ChR2-mCherry (400 nL) was delivered to superficial layers (0.5 mm underneath skull) of somatosensory cortex in right hemisphere at a speed of 1.2 µL/min using syringe pump (Harvard Apparatus, Inc.).
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