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Facscan laser flow

Manufactured by Beckman Coulter
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Sourced in United States

The FACScan laser flow cytometer is a compact and reliable instrument designed for cell analysis. It utilizes a laser to illuminate and detect particles or cells within a fluid stream, allowing for the measurement of various cellular properties such as size, granularity, and fluorescence. The FACScan is capable of performing multiparameter analysis on a diverse range of cell types.

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Lab products found in correlation

Propidium iodide, by Merck Group (2 mentions) Kaluza analysis software, by Beckman Coulter (1 mentions)

Close spelling variants of this product

FACScan laser FACScan

2 protocols using facscan laser flow

1

Cell Cycle Analysis of Propolin C Treatment

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Cell cycle analysis was performed as described previously [31 (link)]. Briefly, cells were seeded and synchronized. After synchronization, propolin C-containing 5% fetal bovine serum medium was incubated for 24 h. Cells were harvested and stained with propidium iodide (50 μg/mL, Sigma-Aldrich, St. Louis, MO, USA), and FACScan laser flow cytometer analysis system (Beckman Coulter, Fullerton, CA) was used to detect cell cycle distribution.
Pai J.T., Lee Y.C., Chen S.Y., Leu Y.L, & Weng M.S. (2018). Propolin C Inhibited Migration and Invasion via Suppression of EGFR-Mediated Epithelial-to-Mesenchymal Transition in Human Lung Cancer Cells. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 7202548.
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2

Cell Cycle Analysis of Propolin G

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MDA-MB-231 cells (2 × 105 cells/well) were cultured and treated with different concentrations of propolin G (5, 10, 15, and 20 μM) for 24 h. Subsequently, cells were collected, washed, and stained with propidium iodide (50 μg/mL, Sigma Chemical, St. Louis, MO, USA). The DNA content was detected by FACScan laser flow cytometer analysis system, and the cell cycle distribution was analyzed by Kaluza analysis software (Beckman Coulter, Fullerton, CA, USA).
Pai J.T., Chen X.H., Leu Y.L, & Weng M.S. (2022). Propolin G-Suppressed Epithelial-to-Mesenchymal Transition in Triple-Negative Breast Cancer Cells via Glycogen Synthase Kinase 3β-Mediated Snail and HDAC6-Regulated Vimentin Degradation. International Journal of Molecular Sciences, 23(3), 1672.
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