To verify the loss of METTL8, cell extracts were loaded onto BOLT 4 to 12% Bis–Tris gels (ThermoFisher) followed by immunoblotting onto PVDF membrane and probing with METTL8 antibody (ThermoFisher, cat. No PA557265, 1:500 dilution) and anti-Actin C4 (EMD Millipore, cat. No MAB1501, 1:000 dilution). Expression of METTL8 in stably infected cell lines were characterized by immunoblotting with the anti-Strep antibody (THE NWSHPQFEK antibody, Genscript, cat. No. A01732, 1:1000 dilution). Transient transfections of TWIN-Strep and FLAG-tagged proteins were also loaded onto BOLT 4 to 12% Bis–Tris gels, immunoblotted to PVDF membranes, and probed with anti-TWIN-Strep (THE NWSHPQFEK antibody, Genscript, cat. No. A01732, 1:1000 dilution), anti-FLAG M2 (anti-FLAG M2, Sigma-Aldrich, 1:3000 dilution), or anti-SARS2 (Abclonal, A12297, 1:1000 dilution). Image analysis of immunoblots was performed using Image Studio software (Li-Cor).
Mettl8 antibody
Manufactured by Thermo Fisher Scientific
The METTL8 antibody is a research tool designed to detect the expression of the METTL8 protein in biological samples. METTL8 is a methyltransferase enzyme involved in the methylation of various cellular components. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of METTL8 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Image studio software, by LI COR (2 mentions)
Anti actin c4, by Merck Group (2 mentions)
Anti flag m2, by Merck Group (2 mentions)
Bolt 4 to 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Nwshpqfek antibody, by GenScript (1 mentions)
Bolt 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
2 protocols using mettl8 antibody
1
Immunoblotting Analysis of METTL8 and Tagged Proteins
2
METTL8 Protein Detection Protocol
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To verify loss of METTL8, cell extracts were loaded onto BOLT 4-12% Bis-Tris gels (ThermoFisher) followed by immunoblotting onto PVDF membrane and probing with METTL8 antibody (ThermoFisher, cat. No PA557265, 1:500 dilution) and anti-Actin C4 (EMD Millipore, cat. No MAB1501, 1:000 dilution). Expression of METTL8 in stably-infected cell lines were characterized by immunoblotting with the anti-Strep antibody (THE TM NWSHPQFEK antibody, Genscript, cat. No. A01732, 1:1000 dilution). Transient transfections of TWIN-Strep and FLAG-tagged proteins were also loaded onto BOLT 4-12% Bis-Tris gels, immunoblotted to PVDF membranes and probed with anti-TWIN-Strep (THE TM NWSHPQFEK antibody, Genscript, cat. No. A01732, 1:1000 dilution) and Anti-FLAG M2 (Anti-FLAG M2, Sigma-Aldrich, 1:3000 dilution). Image analysis of immunoblots were performed using Image Studio software (Li-Cor).
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