Female ICR mice at 36 weeks of age (n = 102) were purchased from Japan SLC Inc. Additionally, Female ICR mice at eight weeks of age (n = 15) were used to evaluate the fertility of young mice. These mice were housed five per cage under a controlled environment (12 h light/dark cycle, temperature 20–25°C and humidity 50%–60%). They were allowed free access to water via sipper bottle. All experimental procedures and animal housing conditions were approved by the Animal Care and Use Committee of the International University of Health and Welfare (Approval Number: 19005). Animal care was consistent with institutional and National Institutes of Health guidelines. To assess the effect of HET on the mice's body mass, all mice were weighed once a week by a digital scale to get the average body weight in each group. In addition, their behavior, fur color, drinking, and eating habit were also noted daily.
Female icr mice
Manufactured by Japan SLC
Sourced in Japan
Female ICR mice are a common laboratory animal model used for various research purposes. They are outbred mice, which means they have a genetically diverse background. The ICR strain is known for its ability to adapt well to different experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Male icr mice, by Japan SLC (3 mentions)
Serotropin, by Aska Pharmaceutical (3 mentions)
M2 medium, by Merck Group (2 mentions)
Gonatropin, by Aska Pharmaceutical (2 mentions)
Male c57bl 6n mice, by Japan SLC (1 mentions)
Optimal cutting temperature compound, by Sakura Finetek (1 mentions)
Biophen fvii kit, by Hyphen Biomed (1 mentions)
Nepa21, by Nepa Gene (1 mentions)
C57bl 6 mice, by Japan SLC (1 mentions)
17 protocols using female icr mice
1
Effects of HET on Aging Mice
2
Generating Transgenic Mice from ESCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eight-week-old ICR female mice (Japan SLC) received 7.5 U of serotropin (ASKA Animal Health) by intraperitoneal injection. Forty-eight hours after serotropin treatment, mice were injected with 7.5 U of gonadotropin (ASKA Pharmaceutical) and then mated with ICR male mice (Japan SLC). Two-cell fertilized eggs were collected by perfusion with mWM medium and maintained in KSOM medium to obtain blastocysts. After injection of six to ten ESCs, the injected blastocysts (22–26 blastocysts/mouse) were transplanted into the uterus of pseudopregnant ICR female mice (Japan SLC). A single ESC line for each genotype was used in this study.
3
Blastocyst Collection and Microinjection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blastocysts collection: 8-week-old ICR female mice (Japan SLC) were treated with 7.5 U of serotropin (ASKA Animal Health) by intraperitoneal injection. After 48 h of serotropin treatment, mice were injected 7.5 U of gonatropin (ASKA Pharmaceutical). These mice were then mated with ICR male mice (Japan SLC). Plug check was performed the next morning. Two days later, these female mice were sacrificed by cervical dislocation and operated to pick out oviducts. Two-cell stage fertilized eggs were collected by perfusion with M2 medium (Sigma) and maintained in KSOM medium. Two days later, the obtained blastocysts were used for microinjection. For microinjection, ESCs were treated with trypsin and pipetted 15 times to dissociate them into single cells. The single cells were cultured in a gelatin-coated 10-cm dish with 10 mL ESC medium for 30 min to attach MEFs onto the dish. Three milliliters of supernatant containing ESCs was collected. Three to five ESCs were injected into the ICR blastocysts under an OLYMPUS IX71 microscope. Twenty to 25 injected blastocysts were transplanted into the uterus of pseudopregnant ICR female mice (Japan SLC).
4
Mouse Housing and Handling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female ICR mice (8-week-old; Japan SLC Inc., Shizuoka, Japan) were housed in groups for a week and used for further experiments. Male C57BL/6N mice (8-week-old; Japan SLC Inc.) were singly
housed and used for experiments at the following ages: 13–23 weeks (YF) and 50–55 weeks (AF). All mice were fed food and water ad libitum and housed in cages with a
temperature maintained at 23 ± 2°C and a 12-h light/dark cycle. The Ethics Committee approved all animal experiments for the Laboratory Animals of Tokyo University of Agriculture (No.
2023010).
housed and used for experiments at the following ages: 13–23 weeks (YF) and 50–55 weeks (AF). All mice were fed food and water ad libitum and housed in cages with a
temperature maintained at 23 ± 2°C and a 12-h light/dark cycle. The Ethics Committee approved all animal experiments for the Laboratory Animals of Tokyo University of Agriculture (No.
2023010).
5
Cadmium Exposure in Female Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female ICR mice (25-30 g, Japan SLC, Shizuoka, Japan) were housed under a 12 hr dark/light cycle and given regular food and water ad libitum. We used female mice in this experiment because previous studies have shown higher sensitivity of female animals than males to Cd toxicity (Brzóska et al., 2005a (Brzóska et al., , 2005b)) . The mice were administered Cd (0, 20, or 50 ppm) via drinking water for 1, 2, and 4 months (n = 4-5). At the time of sacrifice, sections of the kidney tissues were prepared. Half of the kidney tissue was embedded in the Optimal Cutting Temperature (O.C.T.) compound (Sakura Finetek USA; Torrance, CA, USA) and immediately frozen at -80°C. The other half was formalin-fixed and used for section preparation. The contralateral kidney was used to measure Cd concentrations and extract total RNAs. The Experimental Animal Research Committee of Tokushima Bunri University provided ethical approval for the use of laboratory animals in this study (Permit No:25) .
6
Mouse Model for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female ICR mice, 4 weeks of age, were purchased from Japan SLC (Shizuoka, Japan).
The experimental protocols were reviewed and approved by the Hokkaido University Animal Care Committee in accordance with the guidelines for the care and use of laboratory animals.
The experimental protocols were reviewed and approved by the Hokkaido University Animal Care Committee in accordance with the guidelines for the care and use of laboratory animals.
7
ICR Mouse Welfare Experiment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were performed in accordance with the Guidelines of the Institutional Animal Care and Use Committee of the College of Pharmacy, Nihon University, Chiba, Japan. Female ICR mice (age: 7 weeks) were purchased from Japan SLC Inc. (Shizuoka, Japan) and were housed in an air-conditioned specific pathogen-free room (24 ± 2°C) lit from 08:00 to 20:00. Food and water were available ad libitum.
8
FVII Silencing in Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four female
ICR mice (4 weeks of age) were purchased from Japan
SLC (Shizuoka, Japan) and intravenously injected with the siFVII/LNPs.
Plasma FVII activity was measured using a Biophen FVII kit (Hyphen
BioMed, Oise, France).
ICR mice (4 weeks of age) were purchased from Japan
SLC (Shizuoka, Japan) and intravenously injected with the siFVII/LNPs.
Plasma FVII activity was measured using a Biophen FVII kit (Hyphen
BioMed, Oise, France).
9
Ehrlich Ascites Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female ICR mice purchased from Japan SLC, Inc. (Shizuoka, Japan) were inoculated with 5 × 105 ascitic mouse tumor cells (Ehrlich ascites cells from Sumitomo Pharmaceuticals Co., Ltd., Osaka, Japan) about 2 weeks before use. The cells were harvested, washed in PBS, and re-suspended in Eagle’s minimal essential medium with 5% fetal bovine serum. For radioactive labeling, the cells were incubated with 12.5 μCi 3H thymidine per 108 cells at 37°C for 1 hr with gentle agitation. After washing three times in PBS, aliquots (1 mL) of labeled cells at 107 cells/mL were incubated with various concentrations of the test agent for 30 min at 37°C. Test solutions were prepared by serial dilution. The maximum concentration was set at a concentration that is lethal to a majority of the cells or the maximum water soluble concentration.
10
Equalized Growth of ICR Mouse Pups
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female ICR mice (Japan SLC, Inc., Shizuoka, Japan) were maintained under controlled light conditions (14L:10D) and were given food and water ad libitum. The day of birth was designated as Day 0. On Day 1, each mother was left with eight pups to equalize the growth of pups between litters. Our investigations were conducted in accordance with the Animal Care Committee of Nara Women's University.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!