Anti rabbit and anti mouse horseradish peroxidase conjugated secondary antibodies

15d-PGJ₂, 9,10-dihydro-15d-PGJ₂, and biotinylated 15d-PGJ₂ were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Biotinylated 9, 10-dihydro-15d-PGJ₂ was kindly provided from Dr. Young-Ger Suh (Cha University, Seongnam, South Korea). RPMI 1640 medium and fetal bovine serum (FBS) were obtained from Gibco BRL (Grand Island, NY, USA). Antibodies against NRF2, HO-1, VEGF, and actin were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). ZnPP was provided from OXIS International Inc. (Portland, OR, USA). The anti-rabbit and anti-mouse horseradish peroxidase conjugated secondary antibodies were purchased from Zymed Laboratories (San Francisco, CA, USA). The enhanced chemiluminescence (ECL) and [γ-³²P] ATP were supplied from Amersham Pharmacia Biotech (Buckinghamxhire, UK). Antioxidant responsive element (ARE) or GC-mutated ARE-driven luciferase porter plasmid and dominant-negative (DN) NRF2 were kindly provided by Prof. Jeffrey A. Johnson (University of Wisconsin-Madison, WI, USA). The following oligonucleotides were used (core sequence is underlined, and mutated bases are in bold): wild-type ARE, 5′-CTC AGC CTT CCA AAT CGC AGT CAC AGT GAC TCA GCA GAA TC-3′; and GC mutant ARE, 5′-CTC AGC CTT CCA AAT CGC AGT CAC AGT GAC TCA ATA GAA TC-3′. siRNAs directed for NRF2 and HO-1 were produced from Dharmacon, Inc. (Lafayette, CO, USA).