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Rat il 1β elisa kit

Manufactured by USCN
Sourced in China

The Rat IL-1β ELISA kit is a laboratory tool used to quantify the levels of interleukin-1 beta (IL-1β) in rat biological samples. IL-1β is a proinflammatory cytokine that plays a crucial role in the immune response. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of IL-1β in the sample.

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2 protocols using rat il 1β elisa kit

1

Neuroinflammation and Oxidative Stress Assay

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The following reagents and kits were used in the present study: Rabbit polyclonal anti-tyrosine hydroxylase (TH; cat. no. 2792; Cell Signaling Technology, Inc., Boston, MA, USA), rabbit polyclonal anti-OX-42 (cat. no. orb11009; Biorbyt, Cambridge, UK), hydroethidine (Molecular Probes, Eugene, OR, USA), LPS (Sigma-Aldrich, St. Louis, MO, USA), heparin (Qianhong Bio-pharma Co., Ltd., Changzhou, China), SST (ProSpec, East Brunswick, NJ, USA), biotinylated goat anti-rabbit secondary antibody (cat. no. A0277) and horseradish peroxidase (HRP)-labeled streptavidin (Beyotime, Shanghai, China), bicinchoninic acid (BCA) kit (Beyotime), Rat TNF-α ELISA kit, Rat IL-1β ELISA kit and Prostaglandin E2 ELISA kit (PGE2; USCN, Wuhan, Hubei, China).
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2

Measurement of IL-1β in Brain and Serum

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For brain region homogenate, measurement of interleukin 1 beta (IL-1β; proinflammatory cytokine) was estimated using commercially available rat IL-1β ELISA Kit (Wuhan USCN Business Co., Ltd., Wuhan, China) following the manufacturer's protocol. For the serum, quantitative measurement of IL-1β was estimated using commercially available rat IL-1β platinum ELISA Kit (eBioscience, San Diego, USA) following the manufacturer's protocol.
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