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Hcf growth medium

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HCF Growth Medium is a cell culture medium designed to support the growth and maintenance of human cell lines. It contains the necessary nutrients, vitamins, and growth factors required for optimal cell proliferation and survival. The composition of this medium is intended to provide a balanced and standardized environment for cultivating a variety of human cell types.

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Lab products found in correlation

Dmem hams f 12 50 50 mix, by Corning (2 mentions) Hek293 cell, by American Type Culture Collection (2 mentions) Ac16 human cardiomyocyte cell line, by Merck Group (2 mentions) Penicillin streptomycin, by Corning (2 mentions) L glutamine, by Merck Group (1 mentions) Bj fibroblasts, by American Type Culture Collection (1 mentions) Chicken serum, by Bioworld Technology (1 mentions) Fibroblast growth kit low serum, by American Type Culture Collection (1 mentions) Medium 199, by Thermo Fisher Scientific (1 mentions) Fibroblast basal medium, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

3 protocols using hcf growth medium

1

Cell Culture Protocols for Diverse Cell Lines

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HEK293 cells (ATCC CRL-1573) were cultured in Eagle’s Minimum Essential Medium (ATCC, 30–2003), supplemented with 10% FBS (Corning, 35-011-CV) and 1% penicillin/streptomycin (Corning, 30-002-CI). Lenti-X 293T and AAVpro 293T cell Lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with high glucose (4.5 g/L), 4 mM L-glutamine, and 3.7 g/L sodium bicarbonate (Sigma-Aldrich Co., No. D5796); supplemented with 10% fetal bovine serum and 1 mM sodium pyruvate. AC16 human cardiomyocyte cell line (MilliporeSigma, SCC109) was cultured in DMEM/Hams F-12 50/50 Mix (Corning, 10-092-CV), supplemented with 12.5%FBS and 1% penicillin/streptomycin. Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500). Cell lines have been authenticated either by the vendor, or by our group using qPCR of cell-type specific gene markers. All cell lines were tested for mycoplasma contamination at the beginning of the projects and no contamination was observed.
Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2024). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. iScience, 27(3), 109219.
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2

Cell Culture Protocols for HEK293, AC16, and HCF

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HEK293 cells (ATCC CRL-1573) were cultured in Eagle’s Minimum Essential Medium (ATCC, 30–2003), supplemented with 10% FBS (Corning, 35-011-CV) and 1% penicillin/streptomycin (Corning, 30-002-CI). AC16 human cardiomyocyte cell line (MilliporeSigma, SCC109) was cultured in DMEM/Hams F-12 50/50 Mix (Corning, 10-092-CV), supplemented with 12.5%FBS and 1% penicillin/streptomycin. HCF cells (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).
Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2023). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. bioRxiv.
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3

Culturing Primary Human and Chicken Fibroblasts

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Primary adult human dermal fibroblasts (HDF) (ATCC, Manassas, VA) and
human cardiac fibroblasts (HCF) (Cell Applications, San Diego, CA) cells were
purchased at passage 2 from LGC Standards, France and Tebu-bio, France
respectively, and cultured in 5% CO2 in a 37°C incubator. HDF were
maintained in fibroblast basal medium (ATCC, Manassas, VA) with fibroblast
growth kit-low serum (ATCC, Manassas, VA), and HCF were maintained in HCF growth
medium (Cell Applications, San Diego, CA) according to the manufacturers’
instructions. BJ Fibroblasts were purchased from ATCC (Manassas, VA) and
cultured in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin
(Gibco, Grand Island, NW). Chicken valvular interstitial cells (cVICs) were
isolated from the anterior mitral leaflet of chicken embryos at HH40 as
described before18 (link). cVICs were
maintained in Medium 199 (Invitrogen, Waltham,MA) containing 5% of chicken serum
(Bioworld, Philadelphia, PA), 0.1% ITS (Gibco, Grand Island, NW) and 1%
Penicillin-Streptomycin (Gibco, Grand Island, NW).
Kyryachenko S., Georges A., Yu M., Barrandou T., Guo L., Bruneval P., Rubio T., Gronwald J., Baraki H., Kutschka I., Aras K.K., Efimov I.R., Norris R.A., Voigt N, & Bouatia-Naji N. (2021). Chromatin Accessibility of Human Mitral Valves and Functional Assessment of MVP Risk Loci. Circulation research, 128(5), e84-e101.
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