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Glutathione peroxidase gsh px assay kits test kits

Manufactured by Nanjing Jiancheng
Sourced in China

Glutathione peroxidase (GSH-Px) assay kits are laboratory test kits used to measure the activity of the enzyme glutathione peroxidase in biological samples. Glutathione peroxidase is an important antioxidant enzyme that plays a role in protecting cells from oxidative stress.

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4 protocols using glutathione peroxidase gsh px assay kits test kits

1

High-Fat Diet-Induced Diabetic Rat Model

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HFD (65.75% basal chow, 20% sucrose, 10% lard, 3% egg yolk powder, 1% cholesterol, 0.25% pig bile salt) was purchased from Beijing Sibeifu Bioscience Co., Ltd. (Beijing, China). Irbesartan was obtained from Sanofi Winthrop Industrie (France). STZ was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Creatinine (Cr), blood urea nitrogen (BUN), urine protein, superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), and glutathione peroxidase (GSH-Px) assay kits test kits were obtained from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). Rat interleukin (IL)-1β, IL-6, tumor necrosis factor alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kit was obtained from Multi Science Biotechnology Co., Ltd. (Hangzhou, China). Reference standards of ferulic acid, atractylodin, tanshinone, astragaloside, hyperoside, loganin, gallic acid, morroniside, allantoin, oleanic acid, ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, paeoniflorin, vaccarin, pyrrolidinium, leonurine, and catalpol were obtained from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China).
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2

High-Fat Diet-Induced Metabolic Dysfunction Evaluation

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HFD (17.7% sucrose, 17.7% fructose, 19.4% protein and 40% fat) was obtained from Beijing Huafukang Bioscience Co., Ltd. (Beijing, China). Triglyceride (TG), total cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), and glutathione peroxidase (GSH-Px) assay kits test kits were obtained from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). Oil Red O Staining kit was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Rat insulin enzyme-linked immunosorbent assay (ELISA) kit was obtained from Multi Science Biotechnology Co., Ltd. (Hangzhou, China). Reference standards of paeoniflorin, baicalin, saikosaponin A, saikosaponin D, emodin, synephrine, succinic acid, 6-gingerol and oleanolic acid were obtained from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China).
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3

Nuciferine's Effects on Lipid Metabolism

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Nuciferine (C19H21NO2; molecular weight, 295.38 Da; purity ≥ 98%) was purchased from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China). HFD (17.7% sucrose, 17.7% fructose, 19.4% protein, and 40% fat) was obtained from Beijing Huafukang Bioscience Co., Ltd. (Beijing, China). Triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), and glutathione peroxidase (GSH-Px) assay kits test kits were obtained from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). Oil Red O Staining Kit was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Total RNA extraction, first-strand cDNA reverse transcription, polymerase chain reaction (PCR) kits and primers were obtained from TianGen Biotechnology Co., Ltd. (Beijing, China). Rat interleukin (IL)-6, IL-1β, and tumor necrosis factor-alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kits were obtained from Shanghai BlueGene Biotech Co., Ltd. (Shanghai, China).
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4

High-Fat High-Carbohydrate Diet-Induced Metabolic Dysregulation

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High-fat high-carbohydrate (HFHC) diet (65.75% basal chow, 20% sucrose, 10% lard, 3% egg yolk powder, 1% cholesterol, 0.25% pig bile salt) was obtained from Beijing Sibeifu Bioscience Co., Ltd. (Beijing, China). Streptozotocin (STZ) and metformin were purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (Cr), blood urea nitrogen (BUN), superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), and glutathione peroxidase (GSH-Px) assay kits test kits were obtained from Nanjing Jiancheng Biological Engineering Institute (Nanjing, China). Rat insulin, interleukin (IL)-1β, IL-6, tumor necrosis factor alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kit was obtained from Multi Science Biotechnology Co., Ltd. (Hangzhou, China). Reference standards of astragaloside, heterophyllin B, atractylodin, harpagide, berberine, baicalin, puerarin, quercetin, protocatechuic acid and tanshinone II A were obtained from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China).
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