Ultracut

The ultrastructure of mouse trabecular meshwork was examined by TEM followed the procedures described previously^{32 (link)}. Mouse eyeballs were fixed in cold 2.5% glutaraldehyde (EM were then post-fixed in 1% osmium tetroxide (Electron Microscopy Sciences) with 1% potassium ferricyanide (Fisher). They were dehydrated through a graded series of ethanol baths and embedded in Epon (made from dodecenyl succinic anhydride, nadic methyl anhydride, Scipoxy 812 Resin, and 2,4,6-tris(dimethylaminomethyl) phenol (Energy Beam Sciences)). Semi-thin (300 nm) sections were cut on a Reichart Ultracut, stained with 0.5% toluidine blue (Fisher), and examined under a light microscope. Ultrathin sections (65 nm) stained with uranyl acetate (Electron Microscopy Sciences) and Reynold’s lead citrate (Fisher) were examined and photographed at 80 kV on a Jeol 1011 TEM.

The ultrastructure of mouse TM was examined by TEM. Mouse eyeballs were fixed in cold 2.5% glutaraldehyde (EM grade, Taab Chemical) in 0.1 M PBS pH 7.3, rinsed in PBS, and were then post-fixed in 1% osmium tetroxide (Electron Microscopy Sciences) with 1% potassium ferricyanide (Fisher). They were dehydrated through a graded series of ethanol baths and embedded in Epon (made from dodecenyl succinic anhydride, nadic methyl anhydride, Scipoxy 812 Resin and 2,4,6-tris(dimethylaminomethyl)phenol, Energy Beam Sciences). Semi-thin (300 nm) sections were cut on a Reichart Ultracut, stained with 0.5% toluidine blue (Fisher) and examined under a light microscope. Ultrathin sections (65 nm) stained with uranyl acetate (Electron Microscopy Sciences) and Reynold's lead citrate (Fisher) were examined and photographed at 80 kV on a Jeol 1011 transmission electron microscope.