Rabbit anti atg13

Manufactured by Cell Signaling Technology

Rabbit anti-ATG13 is a polyclonal antibody raised in rabbits against human ATG13 (autophagy-related protein 13). ATG13 is a serine/threonine-protein kinase that plays a crucial role in the initiation of autophagy, a cellular process involving the degradation and recycling of cellular components. This antibody is designed for the detection and analysis of ATG13 in various applications.

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Lab products found in correlation

Ab105459, by Abcam (2 mentions) Mouse anti lamp1, by BD (2 mentions) Rabbit anti ulk1, by Cell Signaling Technology (2 mentions) Wortmannin, by Thermo Fisher Scientific (1 mentions) Ab117819, by Abcam (1 mentions) Rapamycin, by Merck Group (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Lysotracker deep red, by Thermo Fisher Scientific (1 mentions) Bafilomycin, by Merck Group (1 mentions) Mouse anti gfp, by Roche (1 mentions) Mouse anti actin, by Proteintech (1 mentions) Rabbit anti fip200, by Proteintech (1 mentions) Rabbit anti fip200, by Merck Group (1 mentions) Rabbit anti vapa, by Proteintech (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Sab4200135, by Merck Group (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Rabbit anti lc3, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Rabbit anti phospho ulk1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-ATG13 ATG13

3 protocols using rabbit anti atg13

Autophagy Protein Immunodetection Assays

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The following antibodies were used for immunostaining or immunoblotting assays: mouse anti-LC3 (MBL, M152-3), rabbit anti-LC3 (2775S; Cell Signaling Technology), rabbit anti-p62 (MBL, PM045), rabbit anti-FIP200 (SAB4200135; Sigma-Aldrich), rabbit anti-FIP200 (17250-1-AP; Proteintech), rabbit anti-ULK1 (8054; Cell Signaling Technology), rabbit anti-Atg13 (13468S; Cell Signaling Technology), rabbit anti-ATL2 (16688-1-AP; Proteintech), rabbit anti-ATL3 (ab117819; Abcam), mouse anti-WIPI2 (ab105459; Abcam), mouse anti-VAPB (66191-1-IG; Proteintech), rabbit anti-VAPA (15275-1-AP; Proteintech), mouse anti-LAMP1 (553792; BD Biosciences), rabbit anti-PTPIP51 (20641-1-AP; Proteintech), rabbit anti-ATG101 (13492; Cell Signaling Technology), rabbit anti-Myc (2278S; Cell Signaling Technology), mouse anti-Myc (M5546; Sigma-Aldrich), mouse anti-GFP (11814460001; Roche), mouse anti-actin (60008-1-IG; Proteintech), and rabbit anti-HA (H6908; Sigma-Aldrich).
The following reagents were used in this study: wortmannin (PHZ1301; Life Technologies), bafilomycin (B1793; Sigma-Aldrich), rapamycin (R0395; Sigma-Aldrich), Lipofectamine 2000 (12566014; Life Technologies), Lipofectamine RNAi MAX (13778150; Life Technologies), and LysoTracker Deep Red (L12492; Thermo Fisher Scientific).

Liu N., Zhao H., Zhao Y.G., Hu J, & Zhang H. (2021). Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. The Journal of Cell Biology, 220(7), e202012091.

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Comprehensive Autophagy Protein Analysis

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The following antibodies were used: rabbit anti-C9ORF72 (sc-138763, Santa Cruz Biotechnology), rabbit anti-SMCR8 (ab121682, Abcam), rabbit anti-WDR41 (sc-137922, Santa Cruz Biotechnology), rabbit anti-ATG101 (SAB4200175, Sigma), rabbit anti-ATG13 (13468, Cell Signaling), rabbit anti-ULK1 (8054, Cell Signaling), rabbit anti–phospho-ULK1 (6888, Cell Signaling), rabbit anti-p62 (5114, Cell Signaling), rabbit anti-RFP (R10367, Invitrogen), mouse anti-LC3 (0231-100/LC3-5F10, Nanotools), rabbit anti-LC3 (PM036, MBL), mouse anti–cathepsin D (AF1029, R&D Systems), rat anti–cathepsin L (MAB9521, R&D Systems).

Yang M., Liang C., Swaminathan K., Herrlinger S., Lai F., Shiekhattar R, & Chen J.F. (2016). A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. Science Advances, 2(9), e1601167.

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Autophagy Pathway Protein Analysis

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Antibodies used were rabbit anti-TFEB (4240S; Cell Signaling Technology), mouse anti-LAMP1 (555798; BD Biosciences), mouse anti-LC3 (M152-3; MBL), rabbit anti-p62 (PM045; MBL), mouse anti-WIPI2 (ab105459; Abcam), rabbit anti-ATG13 (13468S; Cell Signaling Technology), goat anti-CTSD (sc-6486; Santa Cruz), mouse anti-GAPDH (B1034; Biodragon), and rabbit anti-p(Ser10)-H3 (NB21-1091; Novus Biologicals).

Wang Z., Chen D., Guan D., Liang X., Xue J., Zhao H., Song G., Lou J., He Y, & Zhang H. (2022). Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway. The Journal of Cell Biology, 221(5), e202112024.

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