Rat anti mouse ter 119

Manufactured by BD

Sourced in United States

Rat anti-mouse Ter-119 is a monoclonal antibody that binds to the Ter-119 antigen expressed on red blood cells and their precursors in mice. It is commonly used as a marker for the erythroid lineage in flow cytometry and other immunological applications.

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Lab products found in correlation

Rabbit anti desmin, by Abcam (1 mentions) Mouse anti human α sma, by Merck Group (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Rabbit anti mouse lyve 1, by Abcam (1 mentions) Rabbit anti gfp, by Cell Signaling Technology (1 mentions) Hamster anti mouse podoplanin, by Developmental Studies Hybridoma Bank (1 mentions) Goat anti human prox1, by R&D Systems (1 mentions) Goat anti gfp, by Abcam (1 mentions) Rabbit anti α sma, by Abcam (1 mentions) Rabbit anti erg, by Abcam (1 mentions) Alexa fluor 488 conjugated claudin 5 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) A11006, by Thermo Fisher Scientific (1 mentions) Plvap meca 32, by BD (1 mentions) Rat anti mouse, by BD (1 mentions) Isolectin b4, by Thermo Fisher Scientific (1 mentions) Rabbit anti lef1, by Cell Signaling Technology (1 mentions) Click it edu alexa fluor 488 imaging kit, by Thermo Fisher Scientific (1 mentions) Mouse anti human cd19, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

4 protocols using rat anti mouse ter 119

Comprehensive Immunohistochemistry Staining Protocol

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Rat anti-mouse PECAM-1, MEC13.3 (BD Pharmingen), rabbit anti-mouse PECAM-1 (Abcam), mouse anti-human α-SMA, Clone1A4-Cy3 (Sigma), rabbit anti-Desmin (Abcam), rat anti-mouse Ter-119 (BD Pharmingen), rabbit anti-mouse LYVE-1 (Abcam), hamster-anti mouse Podoplanin (Developmental Studies Hybridoma Bank), goat anti-mouse Integrin-α9 (R&D systems), goat anti-human Prox1 (R&D systems), rabbit anti-Prox1 (Angiobio), rat anti-mouse integrin-α5 (BD Pharmingen), goat anti-GFP (Abcam), rabbit anti-GFP (Cell Signalling), rabbit anti-mouse PDGFRβ 28E1 (Cell Signaling), rabbit anti-αSMA (Abcam). Secondary antibodies were Alexa488, Alexa594, and Alexa647 conjugated antibodies (Invitrogen).

Turner C.J., Badu-Nkansah K., Crowley D., van der Flier A, & Hynes R.O. (2014). Integrin-α5β1 is not required for mural cell functions during development of blood vessels but is required for lymphatic-blood vessel separation and lymphovenous valve formation. Developmental biology, 392(2), 381-392.

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Retinal Vascular Development Evaluation

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To evaluate the development of retinal vessels, retinae were dissected from the eyes and fixed in 4% PFA at 4 °C overnight, followed by a rinse in phosphate-buffered saline (PBS). After being blocked in blocking buffer containing 1% fetal bovine serum with 0.25% Triton X-100 for 1 h at room temperature, the retinae were incubated overnight with Isolectin B4 (I21413, Thermo Fisher Scientific, USA), and primary antibodies including rat anti-mouse Ter-119 (553670, BD Biosciences, USA), rat anti-mouse plasmalemma vesicle-associated protein (PLVAP)/MECA-32 (553849, BD Biosciences, USA), rabbit anti-LEF1 (76010, Cell Signaling Technologies, USA), rabbit anti-ERG (ab92513, Abcam, USA), and Alexa Fluor 488-conjugated Claudin-5 monoclonal antibody (352588, Thermo Fisher Scientific, USA). The retinae were washed 3 times in PBS followed by incubation with appropriate Alexa Fluor-conjugated secondary antibodies (A-11006, A32721, or A27018, Thermo Fisher Scientific, USA) for 4 h. The Click-iT EdU Alexa Fluor-488 Imaging Kit (C10337, Thermo Fisher Scientific, USA) was used in combination with ERG antibody to detect EdU positive endothelial cells in retinae of pups that were sacrificed 3 h after intraperitoneal EdU injection.

Li S., Yang M., Zhao R., Peng L., Liu W., Jiang X., He Y., Dai E., Zhang L., Yang Y., Shi Y., Zhao P., Yang Z, & Zhu X. (2022). Defective EMC1 drives abnormal retinal angiogenesis via Wnt/β-catenin signaling and may be associated with the pathogenesis of familial exudative vitreoretinopathy. Genes & Diseases, 10(6), 2572-2585.

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Monoclonal Antibodies for Xenogeneic Cell Analysis

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The following monoclonal antibodies (mAbs) were used for flow cytometry: Mouse anti-human CD19 (Catalog No. 562653, 555412, 341093), CD3 (Catalog No. 563800, 562426, 555341), CD33 (Catalog No. 562854, 555450), CD34 (Catalog No. 348791), CD38 (Catalog No. 340439, 555459), CD4 (Catalog No. 555348), and CD45 (Catalog No. 563204, 641399, 563204); Rat anti-mouse Ter119 (Catalog No. 557915) and CD45 (Catalog No. 563891, 563410) (BD Biosciences). Analyses were performed with FACSAriaIII and FACSCantoII (BD). To obtain cells for xenogeneic transplantation, BV786-conjugated anti-CD3 mAb, BV605-conjugated anti-CD19 mAb, BV421-conjugated anti-CD33 mAb, PE-Cy7-conjugated anti-CD34 mAb, APC-conjugated anti-CD38 mAb, FITC-conjugated anti-CD90 mAb, and PE-conjugated anti-CD45RA mAb were used. For single-cell sorting, a 100 μm nozzle was used.

Saito Y., Mochizuki Y., Ogahara I., Watanabe T., Hogdal L., Takagi S., Sato K., Kaneko A., Kajita H., Uchida N., Fukami T., Shultz L.D., Taniguchi S., Ohara O., Letai A.G, & Ishikawa F. (2017). Overcoming mutational complexity in acute myeloid leukemia by inhibition of critical pathways. Science translational medicine, 9(413), eaao1214.

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Comprehensive Immunophenotyping Using Diverse Antibodies

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The following primary antibodies were employed for immunohistochemistry, IF, and flow cytometry analysis: rat antimouse ICAM-1 (25ZC7) [31] (link), rat anti-mouse ICAM-2 (3C4) (BD Pharmingen), rat anti-mouse vascular cell adhesion molecule (VCAM-1) (2A11.12) [32] (link), rat anti-mouse PECAM-1 (Mec13.3) (BioLegend), rat anti-mouse PSGL-1 (4RA10) [33] (link), rat anti-mouse CD11b/Mac-1 (M1/70) (BD Pharmingen), rat anti-mouse CD4 (GK1.5) (BD Pharmingen), rat anti-mouse CD8 (Lyt2) (BD Pharmingen), rat anti-mouse CD41 (BD Pharmingen), rat anti-mouse CD45 (M1/9) (BD Pharmingen), rat CD45R/B220 (RA3-6B2) (BD Pharmingen), rat anti-mouse F4/80 (BD Pharmingen), rat antimouse 7/4 (AbD Serotec), rat anti-mouse Ly6G (1A8) [34] (link), rat anti-mouse Ter-119 (BD Pharmingen), rabbit anti-mouse Pselectin [35] (link), rabbit pan-laminin (DAKO Cytomation), rabbit Fig. 1 The flow chart summarizes the experimental setup and the number of mice analyzed at different stages of the study. The latter includes eight animals, which had to be excluded from further analysis. Additional 18 mice underwent sham surgery and are not listed here anti-fibrinogen (LSBio), goat anti-mouse CD3ε (Santa Cruz), and biotinylated murine IgG (Vector). Rat anti-human CD44 (huHERMES-1, 9B5) [36] (link), rat, goat, and rabbit IgG were applied as isotype controls.

Enzmann G.U., Pavlidou S., Vaas M., Klohs J, & Engelhardt B. (2018). ICAM-1^null C57BL/6 Mice Are Not Protected from Experimental Ischemic Stroke. Translational stroke research, 9(6).

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