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Povpc bsa

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POVPC-BSA is a laboratory reagent composed of 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) conjugated to bovine serum albumin (BSA). It is used as a chemical standard in various analytical procedures, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Phosphocholine bsa pc bsa, by Biosearch Technologies (2 mentions) Monoclonal anti myc and anti his alkaline phosphatase conjugated antibodies, by Merck Group (2 mentions)

2 protocols using povpc bsa

1

Isolation and Modification of LDL

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Plasma was obtained from healthy donors after an overnight fast following consent under a protocol approved by the UCSD Human Research Protections Program. LDL was isolated by sequential ultracentrifugation, and modified with malondialdehyde (MDA), malondialdehyde-acetaldehyde-adducts (MAA) or CuSO4 to generate MDA-LDL, MAA-LDL or copper-oxidized LDL (Cu-OxLDL) respectively, as previously described31 (link). Phosphocholine-BSA (PC-BSA) was from Biosearch Technologies and POVPC-BSA was prepared as described32 (link). Monoclonal anti-Myc and anti-His alkaline phosphatase (AP) conjugated antibodies were from Sigma.
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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2

Isolation and Modification of LDL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma was obtained from healthy donors after an overnight fast following consent under a protocol approved by the UCSD Human Research Protections Program. LDL was isolated by sequential ultracentrifugation, and modified with malondialdehyde (MDA), malondialdehyde-acetaldehyde-adducts (MAA) or CuSO4 to generate MDA-LDL, MAA-LDL or copper-oxidized LDL (Cu-OxLDL) respectively, as previously described31 (link). Phosphocholine-BSA (PC-BSA) was from Biosearch Technologies and POVPC-BSA was prepared as described32 (link). Monoclonal anti-Myc and anti-His alkaline phosphatase (AP) conjugated antibodies were from Sigma.
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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