Cd16 bv786

Manufactured by BioLegend

The CD16-BV786 is a fluorochrome-conjugated antibody that binds to the CD16 receptor, also known as the Fc gamma receptor III. It is commonly used in flow cytometry applications to identify and characterize natural killer (NK) cells and other immune cell subsets that express the CD16 antigen.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using cd16 bv786

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following pre-conjugated antibodies were used for flow cytometry: CD56-PE (clone 5.1H11), CD56-PC7 (clone 5.1H11), CD56-PacBlue (clone 5.1H11), CD3-FITC (clone OKT3), CD16-BV786 (clone 3G8), CD57-PE (clone HCD57), CD3-APC (clone OKT3), CD3-PacBlue (clone UCHT1), CD25-PE (clone PC61.5), PD-L1-APC (clone 29E.2A3), CD8-PC7(clone RPA-T8), IFNγ-Alexa647(clone 4S.B3), TNFα-PacBlue(clone Mab11) (BioLegend); CD45-APC(clone 2D1), CD45-eFluor450(clone 2D1), PD-1-eVolve655 (clone J105), FoxP3-FITC (clone PCH101), TNFα-eF450 (clone Mab11) (eBioscience); and CD4-APC-H7 (clone RPA-T4) (BD Biosciences). All samples were acquired on a CytoFlex flow cytometer and analyzed using CyteExpert or FlowJo software (v10.0.7).

Oyer J.L., Gitto S.B., Altomare D.A, & Copik A.J. (2018). PD-L1 blockade enhances anti-tumor efficacy of NK cells. Oncoimmunology, 7(11), e1509819.

+ Open protocol

+ Expand

Multiparametric Analysis of CAR-NK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All samples were stained with anti-CD56-APC, anti-CD3-FITC (Biolegend) and 7AAD (BD Biosciences). Transgene expression was detected by flow cytometry on 7AAD⁻ CD56(-APC)⁺ CD3(-PE)⁻ cells (Biolegend). For NK-cell receptor detection, samples were stained with DAPI, CD56-BV711, CD16-BV786, NKp30-AF647, NKp44-PE, NKp46-BV421 (Biolegend), NKG2D-APC (BD Biosciences), and NKG2A-PE (Miltenyi Biotec). CD3-BV650 and CD19-APC-Cy7 (Biolegend) markers were used as a gating exclusion strategy for the NK cell staining. Receptor expression was assessed on DAPI⁻ CD56(-BV711)⁺ CD3(-BV650)⁻ cells. To detect CAR-expression, cells were incubated with 2 μl Siglec2(CD22)-Fc chimera (50 mg/ml, R&D) for 30 min at 4°C, washed and stained with anti-Fc-PE (Jackson Immune).

Colamartino A.B., Lemieux W., Bifsha P., Nicoletti S., Chakravarti N., Sanz J., Roméro H., Selleri S., Béland K., Guiot M., Tremblay-Laganière C., Dicaire R., Barreiro L., Lee D.A., Verhoeyen E, & Haddad E. (2019). Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped Lentivector. Frontiers in Immunology, 10, 2873.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!