Resminostat

Resveratrol, piceatannol, resminostat, entinostat, mocetinostat, vorinostat, curcumin, garcinol, anacardic acid and Tip60i were purchased from Selleckchem (Houston, TX, USA). MB-3 and BMS-345541 were purchased from MilliporeSigma (Burlington, MA, USA). Pterostilbene and myricetin were from LKT Laboratories (St. Paul, MN, USA) and trimethoxy-Resveratrol (trans-3,5,4′-trimethoxystilbene) was from Cayman Chemical Co. (Ann Arbor, MI, USA). Stock solutions of the chemicals were prepared based on the information provided by the manufacturer and maintained at −20°C. The antibodies for human PD-L1 (E1L3N, 13684), p38 MAPK (D13E1, 8690), NF-κB p65 (D14E12, 8242), γH2AX (20E3, 9718), cleaved caspase 3 (D3E9, 9579), IRF-1 (D5E4, 8478) and rabbit IgG isotype monoclonal antibody (DA1E, 5742) conjugated to PE were obtained from Cell Signaling Technology (Beverly, MA, USA). c-Myc antibody (9E10, sc-40) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Fetal bovine serum, RPMI-1640, DMEM, streptomycin and penicillin were from Gibco/Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of analytical grade.

The HH, H9 and HUT78 cell lines were a generous gift from Dr Giandomenico Russo (Istituto Dermopatico dell'Immacolata, IDI–IRCCS, Rome, Italy). The SeAX cell line was a generous gift from Dr Katarzyna Izykowska (Institute of Human Genetics, Polish Academy of Science, Poznan, Poland). 293T cells were purchased from ATCC. The cells were maintained in RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum, L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 µg/ml). HDAC inhibitors (ricolinostat, citarinostat and resminostat), as well as PI3K inhibitors (duvelisib, copanlisib and pictilisib) were purchased from Selleckchem. Inhibitors were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) or 10% trifluoric acid (Sigma-Aldrich; Merck KGaA) in the case of copanlisib.