CA-074Me was purchased from ApexBio (Cat# A8239). Caspase-1 inhibitor Ac-YVAD-CMK was purchased from Cayman (Cat# 10014). Latex beads were purchased from Polysciences.com (Cat#17458–10). LysoTracker™ Red DND-99 was purchased from Thermo Scientific (Cat# L7528). Cathepsin B substrate Z-Arg-Arg-pNA was obtained from Sigma (Cat# SCP0108).
Ca 074me
Manufactured by Apexbio
CA-074Me is a chemical compound used as a laboratory reagent. It functions as a specific and potent inhibitor of the lysosomal cysteine protease cathepsin B.
Automatically generated - may contain errors
Lab products found in correlation
Z arg arg pna, by Merck Group (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Ac yvad cmk, by Cayman Chemical (1 mentions)
Fluorescent dye conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti phospho akt s473, by Cell Signaling Technology (1 mentions)
Plko 1, by Thermo Fisher Scientific (1 mentions)
H1650, by American Type Culture Collection (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti egfr, by Merck Group (1 mentions)
Anti nedd4, by Merck Group (1 mentions)
Anti pten, by Santa Cruz Biotechnology (1 mentions)
Anti flag m2 f1804, by Merck Group (1 mentions)
3 protocols using ca 074me
1
Caspase-1 Inhibition and Lysosomal Trafficking
2
Therapeutic Agents in Preclinical Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ca-074Me (APExBIO; Houston, TX) was diluted in TBS or PBS to 2 mg/ml and dosed intraperitoneally at 25 mg/kg, 6 times a week. Sunitinib malate (clinical grade) was resuspended in citrate buffer (pH 2.3) and administered at 30 mg/kg by daily oral gavage, 6 days per week.
3
Signaling Pathways in Lung Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-NEDD4 was purchased from Millipore (07–049); anti-EGFR (1005) and anti-ACK1 (A11) was from Santa Cruz; anti-PTEN (#9552), anti-AKT (#9272) and anti-phospho-AKT (S473) (#9271) from Cell Signaling; anti-GFP (MMS-118R), anti-HA (MMS-101R) from BioLegend; anti-flag (M2) (F1804) and anti-ACTIN (A5441) from Sigma-Aldrich; anti-EGFR (Mab528) was prepared from culture medium of the EGFR (Mab528) hybridoma cell line (ATCC). The cathepsin B ELISA assay kit was from RayBiotech. Fluorescent dye-conjugated secondary antibodies and phalloidin were purchased from ThermoFisher. The cathepsin B inhibitor CA-074Me was purchased from Apexbio. The NEDD4 shRNA (5’-AUUUGAACCGUAUAGUUCAGC-3′) in the lentiviral expression vector pLKO.1 was purchased from Open Biosystems (RHS4533-EG4734). The lung cancer cell lines A549 and H1650 were purchased from ATCC.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!