Rabbit anti syntaxin1a

The following antibodies were used for western blotting (WB): rabbit anti-ERM (1:1,000; Cell Signaling, Danvers, MA); rabbit anti-PERM (1:2,000; Cell Signaling); mouse anti-α-tubulin (1:15,000; Sigma, Taufkirchen, Germany); rabbit anti-myc (1:4,000; Sigma); mouse anti-PSD-95 (1:2;000; ABR, Golden, CO); mouse anti-gephyrin (BD Bioscience, CA); rat anti-Rdx (R21, 1:50; ref. 77 (link)); rabbit anti-GABA_A receptor α5 (1:1,000, Acris, Herford, Germany); rabbit anti-GABA_A receptor α1 (1:1,000, Serotec, Oxford, UK); mouse anti-β-actin (1:3,500; Sigma); mouse anti-SNAP25 (1:2,000; BD Bioscience); rabbit anti-syntaxin1a (1:1,000; GeneTex, Irvine, CA); peroxidase-conjugated goat anti-rat, goat anti-rabbit and goat anti-mouse (all 1:15,000; Dianova, Hamburg, Germany).
The following antibodies were used for immunofluorescence: rabbit anti-GABA_A receptor α5 (1:50); rabbit anti-GABA_A receptor α2 (1:250; Synaptic Systems, Göttingen, Germany); rabbit anti-VGAT (1:500; Synaptic Systems); mouse anti-synaptic vesicle (SV2, 1:100; Hybridoma Bank, University of Iowa, IA); mouse anti-gephyrin (mab7a, 1:250; Synaptic Systems); rat anti-Rdx (R21, 1:50; ref. 7 (link)); mouse anti-GM130 (clone 35, 1:500; BD Bioscience); CY3-conjugated donkey anti-rabbit, CY3-conjugated donkey anti-rat and CY5-conjugated donkey anti-mouse (all 1:500; Dianova).

The following antibodies were used: mouse anti-Flag M2 (Sigma, A8592), mouse anti-ubiquitin FK2 (Enzo, PW8810), rabbit anti-ubiquitin (Dako, Ely, UK; Z0458), mouse anti-p97 (Fitzgerald, North Acton, USA; 10R-P104A), rabbit anti-VAPA (Epitomics, Burlingame, USA; S1706), mouse anti-FAF1 (Abnova, Taipei City, Taiwan; H00011124-A01), rabbit anti-FAF1 (courtesy of Millipore, Billerica, USA), rabbit anti-GAPDH (Cell Signaling, 2118), rabbit anti-RAB3GAP1 (Proteintech, Manchester, UK; 21663-1-AP), rabbit anti-RAB3GAP2 (Abgent, Maidenhead, UK; AP9635B), rabbit anti-WDR44 (Bethyl, Montgomery, USA; A301-441A), mouse anti-ASNA1 (Abnova, H00000439-M03), rabbit anti-Syntaxin 1A (GeneTex, Irvine, USA; GTX113559), rabbit anti-BAG6 (Cell Signaling, 8523S), mouse anti-RPN2 (Abnova, H00006185-B01), rabbit anti-CD147 (Abcam, Cambridge, UK; ab108317), Alexa Fluor® 488 goat anti-rabbit (Invitrogen, A11008) and Alexa Fluor® 594 chicken anti-mouse (Invitrogen, A21201). Anti-VAPA (Epitomics, S1706) antibodies recognize both VAPA (upper band) and VAPB (lower band) in human cell extracts. The following antibodies were raised and affinity purified using the appropriate antigen by the DSTT: sheep anti-FAF1 (S370D; antigen human FAF1, 1-650), rabbit anti-VAPB (R2986 and R2987; antigen human VAPB, 1-210).