Thermofisher pa1 036

Escherichia coli-derived lipopolysaccharide (LPS) was obtained from InvivoGen (San Diego, CA, USA). Recombinant mouse RANKL and macrophage colony-stimulating factor (M-CSF) were purchased from R&D (Minneapolis, MN, USA). Penicillin, streptomycin, and minimal essential medium alpha (α-MEM) were purchased from Gibco BRL (Gaithersburg, MD, USA). Fetal bovine serum (FBS) was purchased from Avantor (Radnor, PA, USA). The Cell Counting Kit-8 (CCK-8) was obtained from Beyotime Biotechnology (Shanghai, China). Methylisothiourea sulfate (SMT) was purchased from Selleck (Houston, TX, USA). Dihydroethidium (DHE) was purchased from Med Chem Express (Shanghai, China). The Prime Script RT reagent Kit and TB Green^TM Premix Ex Taq^TM II were obtained from Takara Biomedical Technology (Beijing, China). The primary antibody against iNOS (Thermofisher #PA1-036) was purchased from Thermofisher Scientific (Waltham, MA, USA). Primary antibodies against phospho-p65 (CST #3033), p65 (CST #8242), Keap1 (CST #4678), Nrf2 (CST #12721), HO-1 (CST #26416), and NQO1 (CST #62262) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Primary antibodies against β-Actin (Affinity #AF7018), GAPDH (Affinity #AF7021), NFATc1 (Affinity #DF6446), CTSK (Affinity #DF6614), and TNF-α (Affinity #AF7014) were purchased from Affinity Biosciences (Cincinnati, OH, USA).

GLX351322, an inhibitor of NOX4, was obtained from Selleck (Houston, TX, USA). LPS from Escherichia coli O55:B5 was purchased from Sigma-Aldrich (Shanghai, China). Minimal essential medium alpha (α-MEM) was obtained from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Avantor (Gaithersburg, MD, USA). TB Green Premix Ex Taq II and the Prime Script RT Reagent Kit (Perfect Real Time) were obtained from Takara Biotechnology (Otsu, Shiga, Japan). Cell Counting Kit-8 (CCK-8), ROS assay kit (S0033), and Hoechst 33342 (C1022) were purchased from Beyotime Biotechnology (Shanghai, China). Dihydroethidium (DHE) was purchased from MedChem Express (Shanghai, China). Primary antibodies against β-actin (CST #4970), p65 (CST #8242), phospho-p65 (CST #3033), IκBα (CST #4814), phospho-IκBα (CST #2859), p38 (CST #8690), phospho-p38 (CST #4511), ERK (CST # 4695), phospho-ERK (CST #4370), JNK (CST #9252), and phospho-JNK (CST #4668) were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). Primary antibody against iNOS (Thermo Fisher #PA1-036) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibody against NOX4 (Affinity #DF6924) was obtained from Affinity Biosciences (Cincinnati, OH, USA).