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CTX‐2 is a laboratory instrument designed for the automated measurement of biochemical markers. It utilizes immunoassay technology to detect and quantify specific analytes in biological samples. The core function of the CTX‐2 is to provide accurate and reliable analytical results to support clinical decision-making.

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2 protocols using ctx 2

1

Serum Biomarkers of Bone Metabolism in Mice

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Blood was collected from mice by cardiac puncture for analysis of several biochemical parameters. Serum total calcium was determined using Stanbio Laboratory Calcium Liquidcolor (CPC; Boerne, TX, USA; Catalog No. 0150‐250). Serum inorganic phosphate was determined using a Phosphate Colorimetric Assay Kit (BioVision, Inc., Milpitas, CA, USA; Catalog No. K410‐500). Serum C‐terminal telopeptides of types I and II collagen (CTX‐1 and CTX‐2; Immunodiagnostic Systems Inc., Fountain Hills, AZ, USA; Catalog No. AC‐06F1 and AC‐08F1, respectively), as well as N‐terminal propeptide of type I procollagen (P1NP) were determined using the RatLaps EIA kit (Immunodiagnostic Systems Inc., Fountain Hills, AZ, USA; Catalog No. AC‐33F1). Serum intact mouse parathyroid hormone (PTH(1‐84)) was measured using the mouse PTH(1‐84) EIA (Quidel Corp, San Diego, CA, USA; Catalog No. 60‐2305).
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2

Quantification of Murine Inflammatory Markers

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Quantification of murine IL1β, IL6, IL10, osteoprotegerin (OPG) and prostaglandin E2 (PGE2) (R&D Systems), CTX2 (ImmunoDiagnostic Systems, Pouilly en Auxois), and cartilage oligomeric matrix protein (COMP) (MD Bioproducts, Zürich) was performed in sera by specific enzyme-linked immunosorbent assays (ELISA) as recommended by the suppliers.
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