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Anti mouse cd62l apc

Manufactured by Thermo Fisher Scientific
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Anti-mouse CD62L APC is a fluorescently-labeled monoclonal antibody that binds to the CD62L (L-selectin) cell surface antigen on mouse cells. CD62L is involved in the homing of lymphocytes to lymphoid tissues. The APC (allophycocyanin) fluorescent label allows for the detection of CD62L-positive cells by flow cytometry.

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Lab products found in correlation

Anti mouse cd4 pe, by Thermo Fisher Scientific (2 mentions) Anti mouse cd8a fitc, by Thermo Fisher Scientific (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Lsrii system, by BD (1 mentions) Streptavidin pe, by Thermo Fisher Scientific (1 mentions) Facs software, by BD (1 mentions) Mouse lineage panel, by BD (1 mentions) Anti mouse gr1 fitc, by Thermo Fisher Scientific (1 mentions) 7 aad, by BD (1 mentions) 7 add, by BD (1 mentions) Recombinant mouse il 2, by Thermo Fisher Scientific (1 mentions) Anti cd95 jo2, by BD (1 mentions) Anti mouse cd4 percp cyanine 5, by Thermo Fisher Scientific (1 mentions) Anti mouse cd3 fitc, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Actinomycin d, by Merck Group (1 mentions) Sodium butyrate, by Merck Group (1 mentions) Hydrogen peroxide, by Sinopharm (1 mentions)

Close spelling variants of this product

Anti-mouse CD62L conjugated to APC APC-eFluor780-labeled anti-mouse CD62L Anti-CD62L-APC Anti-mouse CD62L CD62L-APC Anti-CD62L CD62L

3 protocols using anti mouse cd62l apc

1

Lymphatic Organ Characterization in Mice

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At week 40, all groups of mice from both studies were euthanized to collect lymphatic organs such as the spleen and lymph nodes. Then, the cell suspensions of these secondary lymphoid organs were labeled with anti-mouse CD4 PE, anti-mouse CD8a FITC, anti-mouse CD45R (B220) FITC, anti-mouse CD62L APC and anti-mouse CD27 APC (eBioscience). The procedure was conducted according to the manufacturer’s protocol [31 ]. The labeled cells were then analyzed by flow cytometric analysis using a BD Accuri® C6 flow cytometer.
Vo T.P., Panicker G., Braz-Gomes K., Parenky A.C., Rajbhandari I., Rajeevan M.S., Unger E.R., D’Souza M.J, & Uddin M.N. (2022). Enhanced Immunogenicity of Adjuvanted Microparticulate HPV16 Vaccines Administered via the Transdermal Route. Pharmaceuticals, 15(9), 1128.
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2

Hematopoietic Stem Cell Mobilization and Neutrophil Analysis

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Whole blood samples were red cell lysed and analyzed using the BD LSRII system with FACS software (BD, Franklin Lakes, NJ).
For enumeration of HSC mobilization (Lin-Sca-1+c-kit+Flt3− or LSKF cells) [7 (link)], cells were stained with Mouse Lineage Panel (BD, Franklin Lakes, NJ), streptavidin PE (eBioscience, San Diego, CA), anti-mouse Sca-1 PE-Cy7 (eBioscience, San Diego, CA), anti-mouse c-kit FITC (eBioscience, San Diego, CA), anti-Flt3 APC (eBioscience, San Diego, CA), and 7-ADD (BD, Franklin Lakes, NJ).
For evaluation of neutrophil L-selectin shedding, cells were stained with anti-mouse Gr-1 FITC (eBioscience, San Diego, CA), anti-mouse CD115 PE (eBioscience, San Diego, CA), anti-mouse CD62L APC (eBioscience, San Diego, CA), and 7-AAD (BD, Franklin Lakes, NJ).
Choi E., Branch C., Cui M.H., Yazdanbakhsh K., Mohandas N., Billett H.H, & Shi P.A. (2015). No evidence for cell activation or brain vaso-occlusion with plerixafor mobilization in sickle cell mice. Blood cells, molecules & diseases, 57, 67-70.
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3

T-Cell Activation and Apoptosis Assay

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TSA, sodium butyrate, propidium iodide, actinomycin D and dimethyl sulfoxide were from Sigma-Aldrich (St Louis, MO, USA). Mouse CD90 (Thy-1) functional grade purified antibody, recombinant mouse IL-2, anti-mouse CD3 FITC, anti-mouse CD4 PErCP-Cyanine 5.5, anti-mouse CD4 PE, anti-mouse CD62L APC, anti-7-AAD PerCP-Cyanine5.5 and anti-mouse CD95 (Fas) PE were from eBiosciences (La Jolla, CA, USA). Anti-CD95 (Jo2) was from BD Pharmingen (San Diego, CA, USA). Mouse CD3 and CD28 polyclonal antibodies were from Abcam (Cambridge, MA, USA). Anti-CTLA4 was generated as described previously.50 (link) Hydrogen peroxide (30%) was from Sinopharm Chemical Reagent (Shanghai, China). SCIENTZ 03-II UV crosslinker was from Scientz Biotechnology (Ningbo, China).
Cao K., Wang G., Li W., Zhang L., Wang R., Huang Y., Du L., Jiang J., Wu C., He X., Roberts A.I., Li F., Rabson A.B., Wang Y, & Shi Y. (2015). Histone deacetylase inhibitors prevent activation-induced cell death and promote anti-tumor immunity. Oncogene, 34(49), 5960-5970.
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