Taqman genotyping assay for

Manufactured by Thermo Fisher Scientific

The TaqMan genotyping assay is a real-time PCR-based technique used for the detection and analysis of genetic variations. It provides a reliable and accurate method for identifying specific DNA sequences or single nucleotide polymorphisms (SNPs) within a given sample.

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Lab products found in correlation

Purelink genomic dna extraction mini kit, by Thermo Fisher Scientific (2 mentions) Amperase ung, by Thermo Fisher Scientific (2 mentions) Cfx96 touch real time pcr detection system, by Bio-Rad (2 mentions) Ds 11 spectrophotometer, by DeNovix (2 mentions) Taqman fast universal pcr master mix, by Thermo Fisher Scientific (2 mentions)

2 protocols using taqman genotyping assay for

TSPO SNP Genotyping from Cerebellar DNA

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Genomic DNA was extracted from cerebellar frozen samples using the PureLink Genomic DNA Extraction Mini Kit (Thermo Scientific, K182002) following manufacturer's instructions. The DNA concentration was measured in a DS‐11 spectrophotometer (DeNovix Inc). TaqMan genotyping assay for the rs6971 SNP in TSPO was purchased from Thermo Scientific (Assay ID C__2512465_20). For the TaqMan assay, DNA samples were diluted to a concentration of 1.8 ng/μL. The polymerase chain reaction (PCR) mix consisted of 1.25 μL of 20× TaqMan SNP genotyping assay, 12.50 μL of 2× TaqMan Fast Universal PCR Master Mix, no AmpErase UNG (Thermo Scientific, 4352042) and 11.25 μL of DNA sample (20 ng), for a total volume of 25 μL per well. DNA samples were run in duplicate. TaqMan assay was run in a Bio‐Rad CFX96 Touch Real‐Time PCR Detection System with the following program: 95ºC × 10 minutes (ramp 1ºC/s), 95ºC × 15 s and 60ºC × 1 minute, for 45 cycles. Principal component analysis of Vic vs. Fam fluorescence (corresponding to base A vs. G) allowed the discrimination between the AA, AG and GG genotypes.

Gui Y., Marks J.D., Das S., Hyman B.T, & Serrano‐Pozo A. (2019). Characterization of the 18 kDa translocator protein (TSPO) expression in post‐mortem normal and Alzheimer's disease brains. Brain Pathology, 30(1), 151-164.

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Genotyping TSPO rs6971 Polymorphism

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Genomic DNA was extracted from cerebellar frozen samples using the PureLink Genomic DNA Extraction Mini Kit (Thermo Scientific, K182002) following manufacturer’s instructions. DNA concentration was measured in a DS-11 spectrophotometer (DeNovix Inc). Taqman genotyping assay for the rs6971 SNP in TSPO was purchased from Thermo Scientific (Assay ID C__2512465_20). For the Taqman assay, DNA samples were diluted to a concentration of 1.8 ng/μL. The polymerase chain reaction (PCR) mix consisted of 1.25 μL of 20x Taqman SNP genotyping assay, 12.50 μL of 2x Taqman Fast Universal PCR Master Mix, no AmpErase UNG (Thermo Scientific, 4352042), and 11.25 μL of DNA sample (20 ng), for a total volume of 25 μL per well. DNA samples were run in duplicate. Taqman assay was run in a Bio-Rad CFX96 Touch Real Time PCR Detection System with the following program: 95°C x 10 min (ramp 1°C/s), 95°C x 15 s, and 60°C x 1 min, for 45 cycles. Principal Component Analysis of Vic versus Fam fluorescence (corresponding to base A versus G) allowed the discrimination between the AA, AG and GG genotypes.

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