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5 protocols using malondialdehyde mda

1

Resveratrol Modulates Inflammation and Oxidation

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Resveratrol (≥99%, Sigma, USA), sodium carboxymethyl cellulose (UNIVAL, Germany), cerulein (Sigma, USA), total cholesterol (TC) and total triglyceride (TG) content assay kits (Solarbio, China), LPS (cusabio, China), MCP-1 (Abcam, USA), TNF-α (Beyotime, China), IL-6 (Abcam, USA) malondialdehyde (MDA) (Solarbio, China), superoxide dismutase (SOD) (Solarbio, China), and total antioxidant capacity (T-AOC) (Solarbio, China) ELISA kits.
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2

Dexmedetomidine Modulates Inflammatory and Oxidative Pathways

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The reagent dexmetomidine was purchased from Jiangsu Hengrui Pharmaceutical Co. Ltd. LPS assay kit was purchased from Sigma (U.S.A.). IL-1β ELISA kit and IL-18 ELISA kit were purchased from Neobioscience Co. Malondialdehyde (MDA) and superoxide dismutase (SOD) were purchased from Solarbio Life Sciences (China, Beijing). IL-1β and IL-18 ELISA kits were also purchased from Solarbio LifeSciences. Cav-1, TLR-4, NLRP3, GAPDH antibody were purchased from Abcam Biotechnology (Britain).
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3

2'-FL Antioxidant Evaluation Protocol

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2′-FL (purity ≥ 98%, cat# GY1141) was purchased from HuicH Technology Co., Ltd. (Shanghai, Beijing, China). Glutathione (GSH, cat: BC1170), malondialdehyde (MDA, cat: BC0020), total antioxidant capacity (T-AOC, cat: BC1310), and superoxide dismutase (SOD, cat: BC0170) were bought from Solarbio Life Science (Beijing, China).
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4

Curcumin-Loaded Nanomedicine for Psoriasis

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Curcumin (CUR) was purchase from Macklin (Shanghai, China). Phosphatidylcholine (PC) was purchased from Lipoid (Ludwigshafen, Germany). Glyceryl Dioleate (GDO) was bought from Shanghai Wencai New Materials Technology Co., Ltd. (Shanghai, China). Propylene glycol (PG), Tween 80, Dialysis bag (molecular weight cut-off: 7000 Da), Hematoxylin and Eosin (H&E) staining kit, Malondialdehyde (MDA) and Superoxide dismutase (SOD) kit were purchased from Solarbio (Beijing, China). IL-17 and IL-22 enzyme-linked immunosorbent assay (ELISA) kits were obtained from Jianglai Biological (Shanghai, China). Indocyanine green (ICG) (MB4675) was bought from Meilun Biotechnology Co., Ltd. (Dalian, China). The anti-Ki67 (ab 15580), anti-VEFG (ab1316) and anti-MPO (ab65871), and donkey anti-rabbit IgG H&L (Alexa Fluor® 647) were obtained from Abcam (MA, US). The anti-CD4 (bs-0647R) and anti-CD8 (bs-0648R) were purchased from BIOSS company (Beijing, China). The anti-cytokeratin 10 (CK 10, AF0197), anti-IL-6 (DF6087) were bought from Affinity Biosciences (OH, USA). The anti-PECAM (sc365804) was provided by Santa Cruz Biotechnology (TX, USA). 5% Imiquimod (IMQ) cream was purchased from Sichuan Ming Xin Pharmaceutical Co., Ltd. (Chengdu, China). Acetonitrile, ethanol, and methanol were all of HPLC grade.
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5

Liver Antioxidant and Enzyme Assessment

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Serum total antioxidant capacity (T-AOC) and activities of alkaline phosphatase (AKP), acid phosphatase (ACP), and superoxide dismutase (SOD) were detected spectrophotometrically by using commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s protocol.
The liver samples were homogenized with ice-cold 0.01 M phosphate buffered saline (PBS, pH: 7.2–7.4; w:v = 1:9), centrifuged at 3000 rpm at 4°C for 10 min and the supernatant was collected. Malondialdehyde (MDA, Beijing Solarbio Science & Technology Co., Ltd.) concentration and activities of glutamic oxaloacetic transaminase (GOT) and glutamic propylic transaminase (GPT) of the supernatant were measured by using commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
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