Tau5 anti tau

Manufactured by Thermo Fisher Scientific

Tau5 anti-tau is a monoclonal antibody that recognizes the tau protein. It is commonly used in research applications to detect and quantify tau levels in biological samples.

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Anti-tau (9 citations)

3 protocols using tau5 anti tau

Immunocytochemical and Western Blot Analysis

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Immunocytochemistry and Western blot analysis were performed as we described before.²⁷, ²⁸ The following primary antibodies were used: anti‐OCT4 (1:200; Santa Cruz), anti‐SOX2 (1:200; Millipore), anti‐NANOG (1:200; R&D Systems), anti‐SSEA‐4 (1:100; Developmental Studies Hybridoma Bank), anti‐TRA‐1‐81 (1:100; Chemicon), Tuj1 anti‐tubulin beta III isoform (1:200; Millipore), anti‐SMA (1:100; DAKO); anti‐AFP (1:100; DAKO), anti‐Nestin (1:200; R&D Systems), anti‐Musashi (1:200; Millipore), anti‐Map2 (1:200; Millipore), anti‐TBR1 (1:100; Abcam), anti‐CTIP2 (1:100; Abcam), Aβ₄₂ anti‐β‐Amyloid₄₂ (1:500; Calbiochem), AT8 anti‐p‐Tau (1:1000; Thermo Fisher Scientific) and anti‐LC3B (1:500; Cell Signaling), Tau5 anti‐tau (1:1000; Thermo Fisher Scientific), anti‐Mfn1 (1:1000; Abcam), anti‐Mfn2 (1:1000; Cell Signaling), anti‐DRP1 (1:1000; Cell Signaling), anti‐Fis1 (1:1000; Santa Cruz), anti‐Ub (1:4000; Santa Cruz), anti‐LAMP2 (1:1000; Santa Cruz), anti‐Beclin1 (1:1000; Cell Signaling), p62 anti‐SQSTM1 (1:1000; Santa Cruz) and anti‐β‐actin (1:10 000; Santa Cruz).

Li L., Kim H.J., Roh J.H., Kim M., Koh W., Kim Y., Heo H., Chung J., Nakanishi M., Yoon T., Hong C.P., Seo S.W., Na D.L, & Song J. (2020). Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F). Cell Proliferation, 53(4), e12798.

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Analysis of Mitochondrial and Autophagy Proteins in Cultured Neurons

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Cultured neurons in 6-well plates were harvested at 10 weeks of differentiation as cell pellets and were resuspended in RIPA buffer (BIOSESANG) containing protease inhibitors (ThermoFisher) and phosphatase inhibitors (ThermoFisher). They were then chilled on ice for 30 min and sonicated. The supernatants were collected after centrifugation at 13,000 rpm for 30 min to remove membrane lipids. Protein concentration was determined using a BCA Protein Assay Kit (ThermoFisher), and 25 ug of protein for each sample was electrophoresed on 8% or 12% gels. The separated samples were transferred onto a PVDF membrane and incubated with target antibodies. Protein bands were visualized by Immobilon Western (Millipore) and were detected using Chemi-Doc (Bio-Rad). The following primary antibodies were used: Tau5 anti-tau (1:1,000, ThermoFisher), AT8 anti-p-tau (1:1,000, ThermoFisher), anti-OPA1 (1:1,000, Santa Cruz), anti-Mfn1 (1:1,000, Abcam), anti-Mfn2 (1:1,000, Cell Signaling), anti-Drp1 (1:1,000, Cell Signaling), anti-Fis1 (1:1,000, Santa Cruz), anti-Ub (1:4,000, Santa Cruz), anti-LC3B (1:1,000, Cell Signaling), anti-LAMP2 (1:1,000, Santa Cruz), anti-Beclin1 (1:1,000, Cell Signaling), p62 anti-SQSTM1 (1:1,000, Santa Cruz), 4G8 anti-APP (1:500, Covance), anti-p-Drp1 (Ser637, 1:1000, Cell Signaling) and anti-β-actin (1:10000, Santa Cruz).

Li L., Roh J.H., Chang E.H., Lee Y., Lee S., Kim M., Koh W., Chang J.W., Kim H.J., Nakanishi M., Barker R.A., Na D.L, & Song J. (2018). iPSC Modeling of Presenilin1 Mutation in Alzheimer's Disease with Cerebellar Ataxia. Experimental Neurobiology, 27(5), 350-364.

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Quantitative Analysis of Neurodegeneration Markers

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Cultured neurons were harvested at 3 weeks of differentiation as cell pellets. Protein samples were prepared and assayed by the method described previously [23 (link)]. The following primary antibodies were used—anti-TDP43 (1:200, Proteintech), anti-FUS/TLS (1:200, Santa Cruz Biotechnology), Tau5 anti-tau (1:1000, Thermo Fisher Scientific), AT8 anti-p-tau (1:1000, Thermo Fisher Scientific), and anti-caspase-3 (1:200, Millipore).

Kim M., Kim H.J., Koh W., Li L., Heo H., Cho H., Lyoo C.H., Seo S.W., Kim E.J., Nakanishi M., Na D.L, & Song J. (2020). Modeling of Frontotemporal Dementia Using iPSC Technology. International Journal of Molecular Sciences, 21(15), 5319.

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