24 well polystyrene tissue culture plates

Manufactured by Corning

The 24-well polystyrene tissue culture plates are a laboratory equipment used for cell culture and other biological experiments. These plates provide a standardized and consistent platform for culturing cells in a 24-well format. The plates are made of polystyrene, a commonly used material for cell culture applications.

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Lab products found in correlation

Ld depletion column, by Miltenyi Biotec (1 mentions) Cd3 microbeads, by Miltenyi Biotec (1 mentions) Floid cell imaging station, by Thermo Fisher Scientific (1 mentions)

2 protocols using 24 well polystyrene tissue culture plates

Expansion and purification of CAR T cells

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Wild-type and gene-modified NK-92 cells were harvested, gamma-irradiated at 30 Gy with a GSR-D1 radiation machine (Gamma-Service Medical GmbH), and resuspended in T-cell medium before use. Cocultures were performed in 24-well polystyrene tissue culture plates (Corning Inc) for optimization studies and in G-Rex 6-well Cell Culture Plates (Wilson Wolf Corporation) for upscaling tests. For the first coculture, CD19-CAR T cells were seeded at 0.5 × 10⁶ per 24-well plate and 10 × 10⁶ per G-Rex 6-well plate. Residual TCR/CD3⁺ T-cell frequencies were assessed every 2 or 3 days via flow cytometry. MACS-mediated TCR/CD3⁺ cell removal was performed using CD3 microbeads and LD depletion columns (Miltenyi).

Kath J., Du W., Martini S., Elsallab M., Franke C., Hartmann L., Drosdek V., Glaser V., Stein M., Schmueck-Henneresse M., Reinke P., Volk H.D., Abou-el-Enein M, & Wagner D.L. (2023). CAR NK-92 cell–mediated depletion of residual TCR+ cells for ultrapure allogeneic TCR-deleted CAR T-cell products. Blood Advances, 7(15), 4124-4134.

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HeLa Cell Cytotoxicity Assay

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HeLa cells were grown as described and seeded at 100,000 cells per well in 24-well polystyrene tissue culture plates (Corning, Corning, NY). After 22 h, medium was changed and a total of 100 ug/ml of CI_01448, CI_01447, 4 ug/ml of TcdA, or PBS were added to each well, and cells were incubated for 24 h. Representative images were acquired using FLoid Cell Imaging Station (Life Technologies Invitrogen, Carlsbad, CA) at 20X magnification. Experiments were performed with at least two biological replicates, each with three technical replicates.

Cherny K., Balaji A., Mukherjee J., Goo Y., Hauser A., Ozer E., Satchell K., Bachta K., Kochan T., Mitra S, & Kociolek L. (2022). Identification of Clostridium innocuum hypothetical protein that is cross-reactive with C. difficile anti-toxin antibodies. Anaerobe, 75, 102555.

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