Quality was checked using an Agilent Tape Station and all samples displayed RIN values above 9. After quantity measurement by Qubit Fluorometer (Invitrogen) 200ng of RNA were used to generate sequencing libraries using the Illumina RNA-Seq Kit V2. Sequencing libraries were subsequently quality checked on D1000 screentapes (Agilent) and 10 samples each were sequenced using a NextSeq550 (Illumina) and 2 NextSeq 500/550 High Output Kits v2.5 (75 Cycles) at the Genomics Core Facility (Ulm University).
Nextseq 500 550 high output kits v2
The NextSeq 500/550 High Output Kits v2 are laboratory equipment designed for high-throughput DNA sequencing. The kits provide the necessary reagents and consumables to perform sequencing runs on the NextSeq 500 or NextSeq 550 platforms. The core function of these kits is to generate high-quality sequencing data by enabling the amplification, detection, and analysis of DNA samples.
Lab products found in correlation
11 protocols using nextseq 500 550 high output kits v2
CD4+ T Cell Isolation and RNA-Seq
Quality was checked using an Agilent Tape Station and all samples displayed RIN values above 9. After quantity measurement by Qubit Fluorometer (Invitrogen) 200ng of RNA were used to generate sequencing libraries using the Illumina RNA-Seq Kit V2. Sequencing libraries were subsequently quality checked on D1000 screentapes (Agilent) and 10 samples each were sequenced using a NextSeq550 (Illumina) and 2 NextSeq 500/550 High Output Kits v2.5 (75 Cycles) at the Genomics Core Facility (Ulm University).
Single-cell RNA-seq of Mesenchymal Cells
Targeted Gene Sequencing from Blood and Buccal Samples
Targeted NGS Sequencing of HBA Genes
Targeted cfDNA and FFPE Sequencing
For genomic DNA from FFPE, libraries were prepared from 2 ng of cfDNA using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA) following the manufacturer’s instructions. Similar to ctDNA libraries, FFPE libraries were pooled before hybridization with the xGen Lockdown probes and sequencing in the Illumina NextSeq 550 system. Both cfDNA and FFPE samples exhibited 5–10% on-target reads.
scRNAseq Analysis of Tumor Immunity
Single-Cell RNA-Seq of Lung Tumor-Infiltrating Leukocytes
scRNA-seq of B[a]P-Induced Lung Tumors
Single-Cell RNA-Seq of B(a)P-Induced Lung Tumors
Tumor-infiltrating Leukocyte Profiling
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