Rat anti lamp 1 1d4b

Manufactured by BD

Sourced in United States, Germany

Rat anti-LAMP-1 (1D4B) is a monoclonal antibody that binds to the lysosome-associated membrane protein 1 (LAMP-1). LAMP-1 is a glycoprotein that is primarily localized in the lysosomal membrane and plays a role in the regulation of lysosomal function and biogenesis.

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Lab products found in correlation

Rabbit anti eea1 h 300, by Santa Cruz Biotechnology (2 mentions) Rat monoclonal anti ha 3f10, by Roche (1 mentions) Mouse monoclonal anti gfp b 2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti actin, by Fortis Life Sciences (1 mentions) Fluorochrome labeled ova, by Thermo Fisher Scientific (1 mentions) Endo h, by New England Biolabs (1 mentions) Pngase f, by New England Biolabs (1 mentions)

3 protocols using rat anti lamp 1 1d4b

Multiparameter Immune Cell Profiling

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Rat anti-MR (MR5D3) and rat anti-CD86 (PO3) were from AbD Serotec; rat anti-LAMP-1 (1D4B) from BD; rabbit anti-EEA1 (H-300) from Santa Cruz; rabbit anti-LY (A5751) from Life Technologies; rat anti-CD8 (53-6.7), rat anti-CD40 (1C10), mouse anti-MHC I (28-14-8) and hamster anti-CD80 (16-10A1) were from eBiosciences and all secondary antibodies from Life Technologies. H-2K^b OVA_257–264 and H-2D^b E7_49–57 tetramers were generated according to standard procedures. Fluorescently labeled antibodies against mouse CD3, CD8, IFN-γ and TNFα were purchased from eBioscience and BD Pharmingen

Rauen J., Kreer C., Paillard A., van Duikeren S., Benckhuijsen W.E., Camps M.G., Valentijn A.R., Ossendorp F., Drijfhout J.W., Arens R, & Burgdorf S. (2014). Enhanced Cross-Presentation and Improved CD8+ T Cell Responses after Mannosylation of Synthetic Long Peptides in Mice. PLoS ONE, 9(8), e103755.

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Immunoblotting for Protein Detection

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CpG oligodeoxynucleotide-1826 (CpG DNA) and biotin-CpG DNA were purchased from TIB Molbiol (Germany). R848 and LPS (Escherichia coli O111:B4) were from Enzo Life Sciences (USA) and Sigma-Aldrich (USA), respectively. Rat monoclonal anti-HA (3F10) was purchased from Roche (USA). Mouse monoclonal anti-GFP (B-2) was purchased from Santa Cruz (USA). Mouse monoclonal anti-myc (9B11), rabbit monoclonal anti-MyD88 (D80F5), mouse monoclonal anti-phospho-Iκβ (5A5), and anti-Iκβ were obtained from Cell Signaling Technology (USA). Rat anti-LAMP1 (1D4B) was obtained from BD Biosciences (USA). Rabbit anti-actin was obtained from Bethyl Laboratories (USA). Rabbit anti-GFP and rabbit anti-myc antibodies were made in our laboratory by immunizing rabbits with purified GFP protein and Myc epitope tag peptide (EQKLISEEDL), respectively.

Song H.S., Park S., Huh J.W., Lee Y.R., Jung D.J., Yang C., Kim S.H., Kim H.M, & Kim Y.M. (2022). N-glycosylation of UNC93B1 at a Specific Asparagine Residue Is Required for TLR9 Signaling. Frontiers in Immunology, 13, 875083.

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Investigating MR-mediated antigen presentation

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Armenian Hamster anti-CD80 (16-10A1), mouse anti-MHC I (28-14-8) and rat anti-CD40 (1C10) were from eBioscience (Frankfurt, Germany), rat anti-MR (MR5D3), mouse anti-His (AD1.1.10) and rat anti-CD86 (PO3) from AbD Serotec (Puchheim, Germany), rat anti-LAMP-1 (1D4B) from BD, rabbit anti-EEA1 (H-300) and all secondary antibodies from Santa Cruz (Heidelberg, Germany).
Fluorochrome-labeled OVA was from Thermo Scientific (Darmstadt, Germany), unlabeled OVA from Sigma (Taufkirchen, Germany), OVA_257-264 and OVA_323-339 from Tebu-bio (Offenbach, Germany), β-gal_497-504 (ICPMYARV) from PSL Peptide (Heidelberg, Germany). PNGase F and Endo H were obtained from New England Biolabs (Frankfurt, Germany).
Wildtype C57BL/6J mice, MR-deficient mice, OT I and OT II mice were bred under specific pathogen-free conditions and used in accordance with local animal experimentation guidelines. Mouse studies were approved by local regulatory agencies (Landesamt fuer Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen - LANUV NRW; Approval number §84-02.04.2012.A264). For all experiments, mice between 8 and 16 weeks of age were used. All mice were anaesthetized before euthanization by cervical dislocation.

Kreer C., Kuepper J.M., Zehner M., Quast T., Kolanus W., Schumak B, & Burgdorf S. (2016). N-glycosylation converts non-glycoproteins into mannose receptor ligands and reveals antigen-specific T cell responses in vivo. Oncotarget, 8(4), 6857-6872.

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