Multi trol mouse

Manufactured by Drew Scientific

The MULTI-TROL Mouse is a laboratory equipment designed for the precise control and monitoring of mouse behavioral parameters. It provides a standardized platform for recording and analyzing mouse activity, movement, and other behavioral metrics in a controlled environment.

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Lab products found in correlation

Vi cell cell viability analyzer, by Beckman Coulter (1 mentions) Heat inactivated bovine serum, by Thermo Fisher Scientific (1 mentions) Hemavet 950, by Drew Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Hemavet hv950, by Drew Scientific (1 mentions)

2 protocols using multi trol mouse

Isolation and Analysis of Hematopoietic Progenitors

Cited 2 times

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Bone marrow cells were isolated by flushing the long bones (femurs and tibias) in Ca²⁺ and Mg²⁺ free Hank’s buffered salt solution (Corning) supplemented with 3% heat-inactivated bovine serum (Gibco). Spleens were prepared by crushing tissues between frosted slides. Cell number and viability were assessed by a Vi-CELL cell viability analyzer (Beckman Coulter) or by counting on a hemocytometer.
Flow cytometric analysis of specific hematopoietic progenitors was performed as previously described (Foley et al., 2013 (link); Signer et al., 2014 (link)). Complete blood cell count analysis was performed on peripheral blood using the Hemavet 950 with MULTI-TROL Mouse as an equilibration control (Drew Scientific).

Mgbemena V.E., Signer R.A., Wijayatunge R., Laxson T., Morrison S.J, & Ross T.S. (2017). Distinct Brca1 mutations differentially reduce hematopoietic stem cell function. Cell reports, 18(4), 947-960.

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Single-Cell Flow Cytometry Analysis of Hematopoietic Cell Populations

Cited 2 times

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For flow experiments, BM and spleen single-cell suspensions were created as described (63 (link)). For HSC and progenitor analysis, cells were incubated with biotinylated lineage specific antibodies (CD3ε, CD4, CD5, CD8a, CD45R, CD11b, Gr-1, Ter119) and fluorophore-conjugated antibodies against progenitor surface markers (Sca1, c-kit, CD16/32, CD34, CD48, CD150). PE-CF594 streptavidin was used to identify lineage-positive cells and DAPI (MilliporeSigma) to exclude dead cells. For lineage analysis, samples were incubated with combinations of fluorophore-conjugated antibodies to the following cell surface markers: CD117, CD3ε, CD4, CD8a, CD45, CD45R, CD11b, Gr-1, Ter119, and CD71. Antibodies used in flow cytometric analysis are included in Supplemental Table 1.
Flow samples were analyzed using the FACS Canto RUO analyzer (BD Biosciences). Gating schemes for hematopoietic progenitors were performed as previously described (17 (link), 63 (link), 64 (link)) using fluorescence minus 1 control. Complete peripheral blood counts were assessed using the HemaVet HV950 with MULTI-TROL Mouse as an equilibration control (Drew Scientific). Blood smear slides were stained using Hemacolor Stain Set (Harleco) according to manufacturer’s instructions.

Lopez-Perez G., Wijayatunge R., McCrum K.B., Holmstrom S.R., Mgbemena V.E, & Ross T.S. (2022). BRCA1 and TP53 codeficiency causes a PARP inhibitor–sensitive erythroproliferative neoplasm. JCI Insight, 7(24), e158257.

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