Magellan standard software version 7

The potential co-effect of nanomaterials and rotating magnetic field on the MCF-7 cell line were examined using Cell Proliferation Reagent WST-1 (Roche Applied Science, Mannheim, Germany) to determine the relative mitochondrial cell activity after incubation with nanomaterials and an 8 h exposure to RMF.
In this experiment, 20 µL of WST-1 solution was added to each well of 96-well microplate and incubated for 60 min at 37 °C. Then, the absorbance values at 450 nm (with a reference wavelength at 630 nm) were measured with a Sunrise Absorbance Reader (Sunrise, Männedorf, Switzerland) equipped with the Magellan Standard Software version 7.2 (Tecan, Männedorf, Switzerland). The cell cultures maintained in complete DMEM medium without nanocomposites served as a negative control. The interaction between the tested nanomaterials and WST-1 reagents were examined using the suspension of nanomaterials at different concentrations in complete DMEM medium incubated for 48 h in standard culture conditions in the absence of cells [21 (link)].

Briefly, the medium of MCF-7 cells incubated for 48 h and additional 8 h exposure to RMF was collected and the cells were washed twice with phosphate-buffered saline (PBS) (Gibco, Langley, OK, USA). Fresh culture medium with 10% neutral red dye (In Vitro Toxicology Assay Kit, Neutral Red based, Sigma-Aldrich, St. Louis, MO, USA) solution was added to the cells and were incubated at 37 °C, 5% CO₂ for another 3 h. Then, the cell cultures were washed twice with PBS and Neutral Red Assay Solubilization Solution (Sigma-Aldrich, St. Louis, MO, USA) was added to the microplates. The cell cultures were incubated for 10 min at room temperature, then were gently stirred for 10 min. The absorbance measurement was performed at a wavelength of 540 nm (background absorbance of Multiwall plates at 690 nm) using a microplate reader (Tecan, Männedorf, Switzerland) equipped with the Magellan Standard Software version 7.2 (Tecan, Männedorf, Switzerland) [21 (link)].