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Estradiol eia kit

Manufactured by Cayman Chemical
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The Estradiol EIA Kit is a laboratory tool used to quantitatively measure the concentration of estradiol, a type of estrogen, in biological samples. It utilizes an enzyme-linked immunosorbent assay (EIA) technique to detect and analyze the target analyte.

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EIA kit (244 citations) Estradiol (11 citations) Estradiol kit (2 citations)

29 protocols using estradiol eia kit

1

Quantification of Snail Hormone Levels

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The hermaphrodite gland tissues of ten snails were employed. Testosterone and estradiol hormone levels were measured for all groups according to manufacturers instructions of the testosterone EIA kit (Enzo Life Science, Michigan, USA, ADI-900-065) and the estradiol EIA kit (Cayman Chemical Company, Michigan, USA, item no. 582251). Briefly, using fresh, disposable tips, 25 µl of each standard, control, and treated samples were dispensed into the proper wells of a microplate coated with either T or E monoclonal antibody. The 200 µl enzyme conjugate was then added to each microplate well, carefully mixed, and allowed to sit at room temperature for 60 minutes. The microplates were then processed, and absorbance was measured (Gholib et al., 2019 (link)).
El-Sayed H., Abdelsalam A., Morad M.Y., Sonbol H., Ibrahim A.M, & Tawfik E. (2024). Phyto-synthesized silver nanoparticles from Sargassum subrepandum: anticancer, antimicrobial, and molluscicidal activities. Frontiers in Plant Science, 15, 1403753.
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2

Influence of Gonadotropins on Ovine Follicle Culture

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The experiments aimed to study the influence of two different gonadotropins (oFSH vs. eCG) on the in vitro follicle culture (ivF) of a single-ovine large PA follicle incubated contextually in FBS-supplemented and FBS-free media. The ivF outcomes were analyzed after 14 days of culture by comparing the follicle/oocyte growth, timing, and percentage (%) of antrum differentiation, as well as the percentage of Metaphase II (MII) oocytes after in vitro Maturation (IVM) and estradiol determination by Estradiol EIA kit (501890; Cayman Chemical Company, Ann Arbor, MI, USA).
Di Berardino C., Peserico A., Capacchietti G., Crociati M., Monaci M., Tosi U., Mauro A., Russo V., Bernabò N, & Barboni B. (2021). Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development. International Journal of Molecular Sciences, 22(22), 12422.
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3

Estradiol Quantification in MCF7 Cells

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The concentration of estradiol in culture medium after 72 h of MCF7 cells incubation with methoxy-stilbenes or resveratrol was assessed by an enzymatic immunoassay according to Jin et al. [21 (link)] using commercial kit (Estradiol EIA Kit, Cayman Chemical Company, Ann Arbor MI, USA) and following the manufacturer’s instructions. Briefly, the assay was based on the competition between estradiol and an estradiol-acetylcholinesterase conjugate for a limited amount of estradiol antiserum. The antiserum-estradiol and antiserum-estradiol-acetylcholinesterase complexes bound to mouse monoclonal anti-rabbit IgG, previously attached to ELISA microplate. Then substrate for acetylcholinesterase was added, and the absorbance of the yellow product of this enzymatic reaction was measured at 405 nm. The concentration of estradiol in medium samples was expressed in pg/mL, using standard curve on each run. The data from three independent experiments were analyzed.
Licznerska B., Szaefer H., Wierzchowski M., Mikstacka R., Papierska K, & Baer-Dubowska W. (2017). Evaluation of the effect of the new methoxy-stilbenes on expression of receptors and enzymes involved in estrogen synthesis in cancer breast cells. Molecular and Cellular Biochemistry, 444(1), 53-62.
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4

Dietary Intervention and Metabolic Assessments

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The diets used in the study were a Teklad custom research diet (catalog # TD.06414) with adjusted calorie diet (60/Fat) containing 18.4% protein, 21.3% carbohydrate and 60.3% fat (referred to herein as high fat diet, HFD), and an isocaloric normal diet 7022 (29% protein, 56% carbohydrate and 15% fat) (referred to herein as normal diet, ND).. Both diets were purchased from Harlan, Indianapolis, IN and contained similar levels of mineral mix AIN-93G-MX (94046) and vitamin mix AIN93-VX (94047). Compound C21 (97% pure) was custom synthesized. The following reagents were purchased from Abcam, MA: plasma free fatty acid quantification assay kit (catalog # ab65341); leptin mouse ELISA kit (catalog # ab100718); CPT-1A antibody (catalog # ab128568); uncoupling protein-1 antibody (catalog # ab10983). The triglyceride quantification assay kit catalog # K622-100 was purchased from Biovision and ultra-sensitive mouse insulin measuring ELISA kit was purchased from Crystal Chem Inc., Downers Grove, IL. The Estradiol EIA kit (catalog # 582251) was purchased from Cayman Chemical, Ann Arbor, MI. The antibodies for AT2R were custom raised from EZ Biolab, Carmel, IN. The anti-β-actin antibody (catalog # 4970) was purchased from Cell Signaling Technology, Danvers, MA.
Nag S., Khan M.A., Samuel P., Ali Q, & Hussain T. (2015). Chronic angiotensin AT2R activation prevents high-fat diet-induced adiposity and obesity in female mice independent of estrogen. Metabolism: clinical and experimental, 64(7), 814-825.
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5

Serum Estradiol Measurement Protocol

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17β-estradiol concentration of serum was measured in both groups (n=5). The blood samples were taken weekly from the tail vein for 5 weeks. The serum was cleared from cellular components of the blood by centrifugation at 12,000 rpm for 10 minutes at 4°C and stored at −80°C until use. The concentrations were measured by using Estradiol EIA Kit (catalog ID: 582251; Cayman Chemical Company, Ann Arbor, MI USA) based on the guidelines of the manufacturer.
Fejes-Szabó A., Spekker E., Tar L., Nagy-Grócz G., Bohár Z., Laborc K.F., Vécsei L, & Párdutz Á. (2018). Chronic 17β-estradiol pretreatment has pronociceptive effect on behavioral and morphological changes induced by orofacial formalin in ovariectomized rats. Journal of Pain Research, 11, 2011-2021.
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6

Plasma 17β-estradiol Quantification

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Blood was obtained from seven rats per group immediately after the experiments. The plasma levels of 17β-estradiol were measured by enzyme linked immunosorbent assay using an Estradiol EIA Kit (Cayman Chemical) according to the manufacturer’s instructions.
Bi R.Y., Meng Z., Zhang P., Wang X.D., Ding Y, & Gan Y.H. (2017). Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ. PLoS ONE, 12(6), e0178589.
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7

Serum Biomarkers of Bone Metabolism

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At 6 weeks of age, the blood was collected at 12:00 (noon). Bone Specific Alkaline Phosphatase (BAP) and tartrate-resistant acid phosphatase 5b (TRACP-5b) were measured using a specific enzyme immunoassay (CUSABIO BIOTECH, Ltd, Wuhan, China). The receptor activator of NF-κB ligand (RANKL) was measured using ELISA kit (R&D SYSTEMS, Minneapolis, MN, USA). Osteocalcin was measured using a MOUSE OSTEOCALCIN EIA KIT (Biomedical Tec Inc., Stoughton, MA, USA). Serum Ca level was measured using a calcium E-test WAKO (WAKO, Tokyo, Japan). β-estradiol (E2) was measured using an Estradiol EIA Kit (Cayman Chemical Co., Ann Arbor, MI, USA). IGF-1 was measured using an Assay Max Mouse IGF-1 ELISA kit (ASSAYPRO, St. Charles, MO, USA). Growth hormone (GH) was measured using a Rat/Mouse Growth Hormone ELISA kit (MILLIPORE, Billerica, MA, USA). Insulin was measured using a sensitive mouse insulin ELISA kit (Shibayagi, Gumma, Japan).
Sasanuma H., Nakata M., Parmila K., Nakae J, & Yada T. (2017). PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis. Molecular Metabolism, 6(5), 428-439.
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8

Estradiol Quantification via Enzyme Immunoassay

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The estradiol concentration was quantified using an enzyme immunoassay kit and following the manufactures guidelines (Cayman Chemical Company, Estradiol EIA Kit, Ann Arbor, Michigan, USA). The kit has previously been used to quantify estradiol in zebra finches (Remage-Healey et al., 2008 (link), Remage-Healey et al., 2012 (link)). The samples of plasma (10–30 µl) were diluted in assay buffer to a final volume of 105 µl, with 50 µl aliquots run in duplicate on a single plate. The plate was read on a Biochrom Anthos 2010 Microplate Reader, ADAP 2.0 (Biochrom Ltd., UK) at a wavelength of 405 nm. Intra-assay coefficient of variation was 9.74% and the detection limit was 6.6 pg/ml.
Emmerson M.G, & Spencer K.A. (2018). Group housing during adolescence has long-term effects on the adult stress response in female, but not male, zebra finches (Taeniopygia guttata). General and Comparative Endocrinology, 256, 71-79.
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9

Estradiol Quantification in Cell Culture

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The 17β-estradiol concentration in spent medium was assayed using estradiol EIA kit as described by manufacturer (Cayman Chemical Company, Ann Arbor, MI 48108).
Malik H.N., Singhal D.K., Saini S, & Malakar D. (2020). Derivation of oocyte-like cells from putative embryonic stem cells and parthenogenetically activated into blastocysts in goat. Scientific Reports, 10, 10086.
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10

Estradiol Quantification in Frozen Serum

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Serum prepared from collected blood samples was -80°C frozen until assayed by Estradiol EIA kit (Cayman) following manufacturer’s instructions. Each sample was analyzed in triplicates. p-value was calculated by t-student test.
Porreca I., D’Angelo F., Gentilcore D., Carchia E., Amoresano A., Affuso A., Ceccarelli M., De Luca P., Esposito L., Guadagno F.M., Mallardo M., Nardone A., Maccarone S., Pane F., Scarfò M., Sordino P., De Felice M, & Ambrosino C. (2014). Cross-species toxicogenomic analyses and phenotypic anchoring in response to groundwater low-level pollution. BMC Genomics, 15(1), 1067.
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