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Deltavision omx sr

Manufactured by GE Healthcare
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The DeltaVision OMX SR is a super-resolution microscope system designed for advanced imaging applications. It utilizes structured illumination microscopy (SIM) technology to achieve resolution beyond the diffraction limit of conventional light microscopes.

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Lab products found in correlation

Softworx 6, by GE Healthcare (3 mentions) Gold grid registration slide, by GE Healthcare (2 mentions) Softworx software, by GE Healthcare (2 mentions) Ab50610, by Abcam (2 mentions) Ab5392, by Abcam (2 mentions) Ab26300, by Abcam (2 mentions) Ab133741, by Abcam (2 mentions) Ab227168, by Abcam (2 mentions) A1r microscope, by Nikon (2 mentions) Lsm 880 airyscan microscope, by Zeiss (2 mentions) Softworx, by Cytiva (2 mentions) Deltavision omx 3d sim imaging system v4, by GE Healthcare (2 mentions) Tirf sim microscope, by GE Healthcare (2 mentions) Planapo, by Olympus (2 mentions) Alexa fluor 647 conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Prolongtm diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) Eclipse ti, by Nikon (1 mentions) Deltavision softworx software, by GE Healthcare (1 mentions) Anti stat3 antibody, by Cell Signaling Technology (1 mentions)

43 protocols using deltavision omx sr

1

Microstructure Characterization by Laser Scanning

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The microstructure was determined according to the methods of Zhou et al. with some modification [22 (link)]. Laser scanning confocal microscopy (Deltavision OMX SR, GE, Fairfield, CT, USA) was used for observation. First, 1 mL sample was diluted 3 times and added to 80 μL of 0.02% Nile Red and 0.1% Nile Blue A staining solution for preparation. The excitation wavelength was 488 nm and 633 nm.
Dou N., Sun R., Su C., Ma Y., Zhang X., Wu M, & Hou J. (2022). Soybean Oil Bodies as a Milk Fat Substitute Improves Quality, Antioxidant and Digestive Properties of Yogurt. Foods, 11(14), 2088.
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2

Localization of Calreticulin in Gold-Treated Cancer Cells

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CAL27 cells (8 × 104 cells per dish) were treated with 50 mg·L−1 Au@C-CCM for 24 h. After 10 min of irradiation, cells were incubated with CRT (1:200, 12238 S, CST) and secondary antibodies. Images were obtained with a GE DeltaVision OMX SR and a confocal microscope. Similar methods were also applied to HN6 and SCC7 cells.
Wu Q., Chen L., Huang X., Lin J., Gao J., Yang G., Wu Y., Wang C., Kang X., Yao Y., Wang Y., Xue M., Luan X., Chen X., Zhang Z, & Sun S. (2023). A biomimetic nanoplatform for customized photothermal therapy of HNSCC evaluated on patient-derived xenograft models. International Journal of Oral Science, 15, 9.
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3

Fluorescence and Confocal Microscopy

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Samples were imaged using either the fluorescence microscopy (Nikon Eclipse Ti, Nikon, Japan) or the confocal microscopy (Olympus FV1000a, Olympus, Japan) with a 100 × /NA 1.45 oil immersion objective. For super resolution imaging, samples mounted in ProLongTM Diamond Antifade Mountant (Invitrogen, Carlsbad, CA, United States) were imaged using a 3D-SIM (Delta Vision OMX SR, GE Healthcare, Chicago, IL, United States).
Hou Y., Wu Z., Zhang Y., Chen H., Hu J., Guo Y., Peng Y, & Wei Q. (2020). Functional Analysis of Hydrolethalus Syndrome Protein HYLS1 in Ciliogenesis and Spermatogenesis in Drosophila. Frontiers in Cell and Developmental Biology, 8, 301.
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4

Multi-modal Microscopy Imaging Protocol

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Widefield-Images were captured using an DeltaVision OMX SR imaging system (GE Healthcare). Images were deconvolved using the DeltaVision SoftWoRx software (GE Healthcare). Stimulated emission depletion (STED) imaging was performed at λ = 775 nm Facility line STED system (Abberior Instruments GmbH, Göttingen, Germany), using a 100x/1.40 oil immersion objective with 561 and 640 nm excitation laser lines at room temperature. Nominal STED laser power was set to ~5–15% of the maximal power of 1200 mW and STED images were acquired with a 25 nm pixel size. Images were deconvolved using Huygens software. Representative still images were chosen. For all images shown, the camera offset value was subtracted and the contrast and brightness were adapted for optimal display of the image. Images are shown in pseudo colors. The Green lookup table (LUT) was used for widefield images, while different channels of super resolution STED images are shown with the LUTs Red Hot (referred to as ‘red’) and Green Fire Blue (referred to as ‘cyan’).
Bou-Nader C., Muecksch F., Brown J.B., Gordon J.M., York A., Peng C., Ghirlando R., Summers M.F., Bieniasz P.D, & Zhang J. (2021). HIV-1 Matrix-tRNA complex structure reveals basis for host control of Gag localization. Cell host & microbe, 29(9), 1421-1436.e7.
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5

Nanoemulsion Particle Characterization

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Microstructural analysis was conducted using a Deltavision OMX SR super-resolution microscope (GE Co., Boston, Massachusetts, USA) to elucidate the particle size and distribution of the nanoemulsions. Based on the work of Liu et al. [27 (link)], 1 mL of the nanoemulsions was mixed with 20 μL of Nile red (0.1%, w/v) and then left in the dark for 30 min after vortex mixing. The stained nanoemulsion samples (5 μL) were deposited on a concave slide with a coverslip. The samples were stored at 4 °C for 12 h before observation.
Zhao S., Wang Z., Wang X., Kong B., Liu Q., Xia X, & Liu H. (2023). Characterization of Nanoemulsions Stabilized with Different Emulsifiers and Their Encapsulation Efficiency for Oregano Essential Oil: Tween 80, Soybean Protein Isolate, Tea Saponin, and Soy Lecithin. Foods, 12(17), 3183.
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6

Immunofluorescence Staining of STAT3

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The CRC or HNSCC cells (5 × 104 cells/well) in a 20 mm confocal dish were treated with DMSO (solvent control) or TSM-1 for different hours, fixed with 4% paraformaldehyde for 20 minutes, and permeabilized with 0.5% Triton X-100 (diluted with water) for 5 minutes. When blocking with 1% BSA (MA0100, Meilunbio) in 1× PBS (MA0015, Meilunbio) for 1 hour at room temperature, cells were stained with anti-STAT3 antibody (1:1000, Cell Signaling Technology [CST]) at 4°C overnight. Following incubation, cells were washed with ice-cold 1× PBS and incubated for another 60 minutes with fluorescently labeled secondary antibody (1:500). The cells were washed with 1× PBS and stained with Hoechst 33342 for 10 minutes. Fluorescence images were visualized using GE DeltaVision OMX SR.
Jin J., Wu Y., Zhao Z., Wu Y., Zhou Y.D., Liu S., Sun Q., Yang G., Lin J., Nagle D.G., Qin J., Zhang Z., Chen H.Z., Zhang W., Sun S, & Luan X. (2022). Small-molecule PROTAC mediates targeted protein degradation to treat STAT3-dependent epithelial cancer. JCI Insight, 7(22), e160606.
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7

High-Resolution Fluorescence Imaging

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SIM images were acquired on a DeltaVision OMX SR imaging system (GE Healthcare, USA) equipped with a 100× oil immersion objective (NA 1.49) and EMCCD, which achieved imaging of samples at approximately 120 nm lateral resolution. Laser lines at wavelengths 488 and 561 nm were used for excitation. The microscope was routinely calibrated with 200 nm diameter fluorescent microspheres. Images were reconstructed with the softWoRx 5.0 software package.
Jin X., Zhang X., Ding X., Tian T., Tseng C.K., Luo X., Chen X., Lo C.J., Leake M.C, & Bai F. (2023). Sensitive bacterial Vm sensors revealed the excitability of bacterial Vm and its role in antibiotic tolerance. Proceedings of the National Academy of Sciences of the United States of America, 120(3), e2208348120.
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8

Mitochondrial Cytochrome c Dynamics

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HeLa cells plated on 20 mm glass plates and treated with RTF for 24 hours were incubated with MitoTracker Red CMXRos (50 nm) for 20 min. After washing with PBS, the cells were incubated with Cyt c antibody (1 : 100) overnight and followed by incubating with the fluorescent-labeled secondary antibody (1 : 1000). After DAPI staining for 10 min, the fluorescence images were captured by using GE DeltaVision OMX SR (GE, USA). Cells without RTF treatment were used as the control.
Gong G., Shen Y.L., Lan H.Y., Jin J.M., An P., Zhang L.J., Chen L.L., Peng W., Luan X, & Zhang H. (2021). The Cyr61 Is a Potential Target for Rotundifuran, a Natural Labdane-Type Diterpene from Vitex trifolia L., to Trigger Apoptosis of Cervical Cancer Cells. Oxidative Medicine and Cellular Longevity, 2021, 6677687.
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9

Immunofluorescence Staining of GFP-Tagged Cells

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Cells were plated and grown on coverslips overnight. After being fixed in 4% paraformaldehyde, cells were treated with 0.5% Triton X-100 for cell permeabilization. The coverslips were then immersed in blocking solution for 1 h, followed by incubation with anti-GFP (T0005, Affinity Biosciences) overnight. Coverslips were washed twice with PBS. Cells were observed and pictures were acquired by fluorescence microscope (Delta Vision OMX SR; GE Healthcare Bio-Sciences, Piscataway, NJ, USA).
Abouelnazar F.A., Zhang X., Zhang J., Wang M., Yu D., Zang X., Zhang J., Li Y., Xu J., Yang Q., Zhou Y., Tang H., Wang Y., Gu J, & Zhang X. (2023). SALL4 promotes angiogenesis in gastric cancer by regulating VEGF expression and targeting SALL4/VEGF pathway inhibits cancer progression. Cancer Cell International, 23, 149.
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10

Superresolution Imaging Using 3D-SIM

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Superresolution 3D structured illumination microscopy (SIM) images were acquired on a DeltaVision OMX SR (GE Healthcare) equipped with a 60× 1.42 NA PlanApo oil immersion lens (Olympus); 405-, 488-, 568-, and 640-nm solid state lasers; and sCMOS cameras (pco.edge). Image stacks with 0.125-µm-thick z-sections, and 15 images per optical slice (three angles and five phases) were acquired using immersion oil with a refractive index 1.516. Images were reconstructed using Wiener filter settings of 0.003 and optical transfer functions (OTFs) measured specifically for each channel with SoftWoRx 6.5.2 (GE Healthcare) to obtain superresolution images with a twofold increase in resolution both axially and laterally. Images from different color channels were registered using parameters generated from a gold grid registration slide (GE Healthcare) and SoftWoRx 6.5.2 (GE Healthcare).
Lim A., Rechtsteiner A, & Saxton W.M. (2017). Two kinesins drive anterograde neuropeptide transport. Molecular Biology of the Cell, 28(24), 3542-3553.
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