Sections were blocked with 2.5% normal horse serum and incubated overnight at 4 °C in primary antibodies as follows: Egr-1 and phosphorylated c-Jun N-terminal kinase (p-JNK) from Cell Signaling, aquaporin-1 (AQP1) from Santa Cruz Biotechnology (Dallas, TX, USA), and Calbindin from Abcam (Cambridge, MA, USA). After washing, the sections were incubated with Alexa Fluor488-conjugated and/or Alexa Fluor594-conjugated secondary antibodies (Vector Laboratories, Burlingame, CA, USA). Fluorescence was visualized using a Fluoview 1000 IX-81 confocal microscope (Olympus). The fluorescence intensity of p-JNK was analyzed using Image J software, Version 1.52a (NIH, Bethesda, MD, USA).
Aquaporin 1 aqp1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Aquaporin-1 (AQP1) is a protein that functions as a water channel in the cell membrane. It facilitates the rapid passage of water molecules across the cell membrane while restricting the passage of other solutes. AQP1 is widely expressed in various tissues and plays a crucial role in maintaining water homeostasis within the body.
Automatically generated - may contain errors
Lab products found in correlation
Calbindin, by Abcam (1 mentions)
Alexa fluor594 conjugated secondary antibodies, by Vector Laboratories (1 mentions)
Fluoview 1000 9 81 confocal microscope, by Olympus (1 mentions)
Vimentin, by Agilent Technologies (1 mentions)
Fibronectin, by Abcam (1 mentions)
Histofine simple stain max po, by Nichirei Biosciences (1 mentions)
α smooth muscle actin α sma, by Agilent Technologies (1 mentions)
Type 4 collagen, by Abcam (1 mentions)
2 protocols using aquaporin 1 aqp1
1
Immunofluorescence Staining of Signaling Proteins
2
Immunohistochemical Analysis of Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formalin-fixed paraffin-embedded sections were autoclaved at 121°C for 15 min in 10 mM
citrate buffer (pH 6.0) for antigen retrieval. The sections were then incubated in 3%
H2O2/methanol to inactivate endogenous peroxidase activity,
followed by incubation with 10% normal goat serum at room temperature for 30 min.
Subsequently, the sections were incubated with antibodies against CD44 (1:1,000, rabbit
polyclonal; Abcam, Cambridge, UK), Ki67 (1:500, rabbit polyclonal; Abcam), α-smooth muscle
actin (αSMA; 1:50, Agilent Technologies, Santa Clara, CA, USA; clone: 1A4), aquaporin-1
(AQP1; 1:300; Santa Cruz, Dallas, TX, USA; clone: B-11), vimentin (1:200; Agilent
Technologies; clone: V9), collagen type IV (1:400, rabbit polyclonal; Abcam), and
fibronectin (1:800, rabbit polyclonal; Abcam) at 4°C overnight. Following this, secondary
antibody reactions were performed using a horseradish peroxidase (HRP)-conjugated antibody
(Histofine Simple Stain MAX PO; Nichirei Bioscience Inc., Tokyo, Japan).
3,3′-Diaminobenzidine tetrahydrochloride was applied for visualization.
citrate buffer (pH 6.0) for antigen retrieval. The sections were then incubated in 3%
H2O2/methanol to inactivate endogenous peroxidase activity,
followed by incubation with 10% normal goat serum at room temperature for 30 min.
Subsequently, the sections were incubated with antibodies against CD44 (1:1,000, rabbit
polyclonal; Abcam, Cambridge, UK), Ki67 (1:500, rabbit polyclonal; Abcam), α-smooth muscle
actin (αSMA; 1:50, Agilent Technologies, Santa Clara, CA, USA; clone: 1A4), aquaporin-1
(AQP1; 1:300; Santa Cruz, Dallas, TX, USA; clone: B-11), vimentin (1:200; Agilent
Technologies; clone: V9), collagen type IV (1:400, rabbit polyclonal; Abcam), and
fibronectin (1:800, rabbit polyclonal; Abcam) at 4°C overnight. Following this, secondary
antibody reactions were performed using a horseradish peroxidase (HRP)-conjugated antibody
(Histofine Simple Stain MAX PO; Nichirei Bioscience Inc., Tokyo, Japan).
3,3′-Diaminobenzidine tetrahydrochloride was applied for visualization.
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