As described in our previous studies (S. Zhu et al., 2017 (link); S. Zhu et al., 2020 (link)), biotin-labeled double strand DNA fragment (dsDNA, 300 bp) was generated using biotin-11-ddUTP (Thermo Scientific) incorporation. Biotin-labeled DNA (produced as above) or biotin dA-dT (Invitrogen) was conjugated on streptavidin magnetic beads (New England Biolabs) and incubated in Xenopus egg extracts or HeLa cell lysates. The beads were re-isolated using a magnet rack, washed five times, and resolved in SDS-PAGE gels, and submitted for mass spectrum analysis at the Taplin mass spectrometry facility, Harvard.
Biotin 11 ddutp
Manufactured by Thermo Fisher Scientific
Biotin-11-ddUTP is a nucleotide analog used in molecular biology applications. It serves as a precursor for the incorporation of biotin labels into DNA or RNA during enzymatic synthesis.
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Lab products found in correlation
2 protocols using biotin 11 ddutp
1
Biotin-labeled DNA Pulldown Assay
2
Biotin-labeled DNA Pulldown Assay
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Biotin-labeled double strand DNA fragment (dsDNA, 500 bp) was generated using biotin-11-ddUTP (Thermo Scientific, #R0081) incorporation, and PCR amplification using Taq polymerase and a pMBP vector (as template). Biotin-labeled DNA (produced as above) or biotin dA-dT (70 mer) was conjugated on streptavidin magnetic beads (New England Biolabs, #S1420S) and incubated in Xenopus egg extracts and HeLa cell lysates. The beads were re-isolated using a magnet, washed five times, and then resolved by SDS-PAGE.
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