Injectable soya lecithin

1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[(polyethylene glycol)₂₀₀₀]-NH₂ (DSPE-PEG-NH₂, molecular weight: 2 kDa) was provided by Pengsheng Biological Co., Ltd. (Shanghai, People's
Republic of China). HA (molecular weight: 3 kDa) was obtained from Shandong Freda Biopharm Co., Ltd. (Ji’nan, People's
Republic of China). Injectable soya lecithin (ISL) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Compritol^® 888 ATO and Glycerol Monostearate (GMS) were obtained as gifts from Gattefossé (Saint-Priest, Lyon, France). RVC hydrochloride, DMDT hydrochloride, N,N-dimethylformamide (DMF), polysorbate 80, DMEM, and MTT were purchased from Sigma Aldrich (St. Louis, MO, USA). All the other chemicals and reagents were of analytical grade or HPLC grade and used without further purification.

1-Ethyl-3(3-dimethylaminopropyl)carbodiimide (EDC), Nhydroxysuccinimide (NHS), dimethylformamide (DMF) were provided by Aladdin Reagent Database Inc (Shanghai, China). Miglyol^® 812 was purchased from Caelo (Hilden, Germany). Compritol^® 888 ATO (ATO) and glycerol monostearate (GMS) were obtained from Gattefossé (Saint-Priest, Lyon, France). Injectable soya lecithin (ISL) was purchased from Lipoid GmbH (Ludwigshafen, Germany). lidocaine (LID), and Pluronic 68 (P68) were purchased from Sigma-Aldrich (St Louis, MO). TAT-PEG-DSPE was obtained from Xi’an Ruixi Biological Co., Ltd (Xi’an, China) Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Invitrogen Corporation (Carlsbad, CA). L929 cell line from mouse (L929 cells) was procured from the American Type Culture Collection (Manassas, VA) and cultured in DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) medium. Kunming mice (6 to 8-weeks-old) and Wistar rats (9 to 11-weeks-old) were procured from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China), and animal handling methods got permission from the Medical Ethics Committee of Shandong Cancer Hospital and Institute.

LID, PRI, DMEM, MTT and dimethyldioctadecylammonium bromide (DDAB; purity ≥98.0%), were purchased from Sigma-Aldrich (St Louis, MO, USA). Injectable soya lecithin (ISL) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Compritol^® 888 ATO, Precirol^® ATO 5 and glycerol monostearate (GMS) were obtained as gifts from Gattefossé (Lyon, France). All other chemicals and reagents were of analytical grade or high-performance liquid chromatography (HPLC) grade and were used without further purification.