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Rabbit anti cd2ap

Manufactured by Santa Cruz Biotechnology
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Rabbit anti-CD2AP is a primary antibody that recognizes the CD2-associated protein (CD2AP), a multifunctional adapter protein involved in actin cytoskeleton organization and cell signaling. This antibody can be used for the detection and analysis of CD2AP expression in various cell and tissue types.

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Lab products found in correlation

Sp2 confocal microscope, by Leica (1 mentions) Cas block, by Thermo Fisher Scientific (1 mentions) Chemmate, by Agilent Technologies (1 mentions) Hoechst fluka, by Merck Group (1 mentions) Confocal microscope, by Bio-Rad (1 mentions) Normal rabbit serum, by Merck Group (1 mentions) Goat antirabbit igg fitc conjugate, by Merck Group (1 mentions) Alexa fluor 488 goat anti guinea pig, by Thermo Fisher Scientific (1 mentions) Rabbit anti gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit anti sumo1, by Cell Signaling Technology (1 mentions) Rabbit anti nephrin, by Santa Cruz Biotechnology (1 mentions) Guinea pig anti nephrin, by Progen Biotechnik (1 mentions)

Spelling variants of this product

Anti-CD2AP (5 citations) Rabbit anti-TM (2 citations)

3 protocols using rabbit anti cd2ap

1

Immunofluorescence Staining of Rat Kidney

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Rat kidneys were snap-frozen and embedded in Tissue-Tek® O.C.T. Compound (Sakura). Cryosections were fixed with 3.5% PFA, permeabilized with 0.1% Triton X-100 in phosphate-buffered saline (PBS) at RT for 15 min and blocked with CAS-block (Invitrogen). Sections were incubated with rabbit anti-CD2AP (Santa Cruz Biotechnology), quinea pig anti-nephrin IgGs (Sigma Aldrich) and goat anti-SHIP2 I-20 IgGs (Santa Cruz Biotechnology) overnight at +4 °C in ChemMate (Dako Cytomation, Glostrup, Denmark), followed by Alexa Fluor 555 donkey anti-rabbit, Alexa Fluor 488 donkey anti-quinea pig and Alexa Fluor 555 donkey anti-goat IgGs (Invitrogen) together with Hoechst (Fluka, Sigma Aldrich) for 1 h in ChemMate (Dako Cytomation). Samples were mounted in Mowiol and examined with Leica SP2 confocal microscope (Leica Microsystems CMS GmbH, Mannheim, Germany).
Saurus P., Tolvanen T.A., Lindfors S., Kuusela S., Holthöfer H., Lehtonen E, & Lehtonen S. (2017). Inhibition of SHIP2 in CD2AP-deficient podocytes ameliorates reactive oxygen species generation but aggravates apoptosis. Scientific Reports, 7, 10731.
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2

Podocyte Response to LN Patient Plasma

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Podocytes were cultured on sterilised coverslips in medium containing plasma from LN patients or from healthy controls. After 48 h exposure of the podocytes to plasma, the effect on the localisation of α-actinin and CD2AP was determined by immunofluorescent staining. Cells were fixed with paraformaldehyde and permeabilised with triton-X 100. Non-specific binding was blocked with 2%FCS/2%BSA/0.1%Tween/PBS containing 10% normal rabbit serum (sigma), and then cells were incubated with the primary antibodies (rabbit-anti-α-actinin-4 (ImmunoGlobe), rabbit-anti-CD2AP (Santa Cruz Biotechnology)). After washing, goat antirabbit IgG-FITC conjugate (Sigma) was added and the cover slips were mounted on glass slides using Mowiol mountant. The slides were viewed on a Biorad confocal microscope and images captured using LaserSharp 2000 and analysed using confocal assistant V.4.02 software.
Manson J.J., Mills K., Jury E., Mason L., D'Cruz D.P., Ni L., Saleem M., Mathieson P., Isenberg D, & Rahman A. (2014). Pathogenic autoantibodies from patients with lupus nephritis cause reduced tyrosine phosphorylation of podocyte proteins, including tubulin. Lupus Science & Medicine, 1(1), e000013.
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3

Antibody Panel for Molecular Analysis

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The antibodies used for Western blot analysis, immunoprecipitation, immunofluorescence, and immunohistochemistry were as follows: rabbit anti-nephrin (targeting for the extracellular domain), rabbit anti-CD2AP, rabbit anti-GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-ubiquitin (Imgenex, San Diego, CA), mouse anti-CIN85 (Upstate), rabbit anti-SUMO-1 (Cell Signaling), guinea pig anti-nephrin (Progen, Heidelberg, Germany), and mouse anti-CIN85 (gift from I.D.). Peroxidase-conjugated donkey anti-rabbit, goat anti-mouse, and unconjugated rabbit IgG were from Santa Cruz Biotechnology. Alexa Fluor 488 goat anti-guinea pig and Alexa Fluor 555 donkey anti-mouse antibodies were from Invitrogen.
Teng B., Schroder P., Müller-Deile J., Schenk H., Staggs L., Tossidou I., Dikic I., Haller H, & Schiffer M. (2016). CIN85 Deficiency Prevents Nephrin Endocytosis and Proteinuria in Diabetes. Diabetes, 65(12), 3667-3679.
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