Uc90 camera

Gonadal samples were fixed in 10% formalin, processed, and embedded into paraffin blocks. The 3 µm serial sections were stained with hematoxylin and eosin. The slides were analyzed on an Axio Lab.A1 microscope (Carl Zeiss Microscopy, Jena, Germany) equipped with an Axiocam ERc5s digital camera (Carl Zeiss Microscopy, Jena, Germany) or on Olympus BX53 light microscope coupled with an Olympus UC90 camera.

Barite-hosted fluid inclusions were analysed on double-polished wafers (0.2 mm thick) by using both Linkam FTIR600 stage mounted on the ZEISS AxioScope A1 microscope with magnification objectives of 10 × , 50 × , and 100 × , equipped with QImaging Micro Publisher 5.0 RTV camera and Linkam THMSG600 stage mounted on the Olympus microscope BX53 with magnification objectives of 5 × , 10 × , 20 × , 50 × , and 100 × equipped with Olympus UC90 camera. The first piece of equipment was available for studies at the Slovak Academy of Sciences in Banska Bystrica, the second at the Faculty of Geology, Geophysics and Environmental Protection AGH University of Science and Technology in Krakow. In both cases, the calibration of the stage was carried out using natural inclusions of pure CO₂, and chemical compounds with known temperatures of phase transitions. The fluid inclusions were subjected to temperatures in the range from − 180 to + 250 °C. The heating runs were made at the rate of 5 °C/min until the final ice melting temperature was approaching.
Samples devoid of two-phase inclusions at room temperature were previously cooled at 5 °C for 24 h in the fridge and at 0.1 °C for several minutes in the freezing-heating stage to induce the vapor nucleation.

The mucosal damage was measured using the macro- and microscopic examination. The stomach from each mouse was removed, opened along the greater curvature, and cleaned gently by dipping in normal saline. The severity of macroscopically visible changes in the mucous membrane was assessed using the semiquantitative 0–3 scale based on the previously described criteria taking into account the presence or absence of gastric erosions, ulcers, and their extent, described in detail in the legend of Table 1 [44 (link)]. Then, stomach specimens demonstrating macroscopic injury of the greater extent collected from each mouse pretreated with studied compound or reference drug, or equivalent stomach specimens from control mice, were selected for the histological assessment. Selected specimens were fixed in 4% buffered formalin, embedded in paraffin, and cut into 4 µm-thick slices, which were mounted on the glass slides and stained by the routine hematoxylin-eosin (H&E) method. Next, the microscopic analysis was performed using an Olympus BX53 light microscope combined with an Olympus UC-90 camera (Olympus, Germany). Histopathological changes of all stomach specimens were examined in a blinded way by the experienced pathologist using a 0–3 point scale.

An Olympus BX53M polarizing microscope (POM) equipped with a Linkam hot stage and an Olympus UC90 camera was used to observe the optical mesophase textures of complex Zn/Fe. Thermal decomposition was carried out using a TGA/SDTA 851-LF 1100 Mettler Toledo thermogravimetric analyser, with the experiments conducted in a nitrogen atmosphere in the temperature range of 25–800 °C with a heating rate of 10 °C min⁻¹. Enthalpies and transition temperatures were recorded using a Q1000 apparatus from TA Instruments. The apparatus was calibrated with indium; three heating/cooling cycles were performed for each sample, with a heating and cooling rate of 10 °C/min.

To check whether extrusion increased the homogeneity of the materials, VHMWPE particles, which are suspended within—and are immiscible with—were tested in the polymer matrix. For this analysis, the extruded samples were cut by a Leica Rotation Microtome RM2265 into 12 µm-thick slices that were embedded in (Eukitt) resin between glass and cover slide. The resin was left to harden for 10 h, and then an Olympus SZX10 stereomicroscope equipped with a UC90 camera (Figure 3) was used to capture images at 10× and 20× magnification.

Tissue samples were embedded in optimal cutting temperature (OCT) compound (Scigen Scientific, Gardena, CA, USA) and snap-frozen in liquid nitrogen. Cryosections of 6 µm thickness were cut with a cryostat (Leica CM3050S) and stored at −80 °C. Transglutaminase activity was localized with an in situ assay according to a published protocol [28 (link)], with minor modifications. Briefly, cryosections were incubated with 5 µM Alexa-fluor-555-cadaverine (Thermo Fisher, Waltham, MA, USA), a substrate of transglutaminases, in 0.1 M Tris-HCl pH 7.4 in the presence of 5 mM CaCl₂ for 2 h. In negative control experiments, CaCl₂ was replaced by 5 mM EDTA. The reaction was stopped by incubating the slides in 25 mM EDTA in PBS for 5 min. Nuclei were labeled with 1 µg/mL Hoechst 33258 (Molecular Probes, Eugene, OR, USA). The sections were mounted with Permafluor (Thermo Fisher, Waltham, MA, USA) and examined with an Olympus BX63 microscope. Photographs were taken with an Olympus UC-90 camera.